Research article summary (published 17 Jan 2006):

Comparative assessment of kaempferitrin from medicinal extracts of Bauhinia forficata link.

Full Abstract

The leaves of the pantropical genus Bauhinia (Fabaceae) are popularly known as cow's-paw or cow's hoof due to their unique characteristic bilobed aspect. The species Bauhinia forficata (Brazilian Orchid-tree) is widely used in folk medicine as an antidiabetic. This article deals with the quantitative analysis of kaempferitrin from B. forficata medicinal extract (aqueous and hydro alcoholic) using the LC method, and the comparison of kaempferitrin content in leaves collected from two different regions in the south Brazil. The total flavonoid content assessed by LC was also compared with the classical spectrophotometric determination. Kaempferitrin was found in different amounts, in samples from two geographical areas (Telêmaco Borba/PR and Itajaí/SC), for aqueous (368.68 and 77.91 microg/mL) and hydro alcoholic extracts (1952.59 and 211.61 microg/mL), respectively. The method was subjected to recovery assay, to determine its accuracy. A marked difference in total flavonoid concentration was observed in relation to kaempferitrin content: 2759.95 and 2188.20 microg/mL for the fluidextract and 863.35 and 856.77 microg/mL for the aqueous extract (Telêmaco Borba/PR and Itajaí/SC). The spectrophotometric assay overestimated the total flavonoid content (3620 microg/mL) in relation to the LC assay.

Author information

Author/s: Pinheiro, Thiátira S D B (TS); Johansson, Loana A P (LA); Pizzolatti, Moacir G (MG); Biavatti, Maique W (MW);

Affiliation: Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Curso de Farmácia, CCS, University of Vale do Itajaí (Univali), Rua: Uruguai 458, Caixa Postal 360, Itajai, Santa Catarina (SC) 88302-202, Brazil.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal), published in England. (Language: eng)

Reference: 2006-May; vol 41 (issue 2) : pp 431-6

Dates: Created 2006/04/17; Completed 2007/08/01;

PMID: 16423486, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Bauhinia - chemistry Brazil Chromatography, Liquid Kaempferols - analysis; isolation & purification Plant Extracts - chemistry

Plant Extracts - chemistry Plant Leaves Plants, Medicinal - chemistry Reproducibility of Results Spectrophotometry, Ultraviolet

Associated Chemicals: Kaempferols (0) ; Plant Extracts (0) ; lespenefril (482-38-2)

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