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| Research article summary (published 17 Apr 2006): |
Electrofocusing of methanolic extracts for identification of individual flavonol biomolecules in Camellia species.
Full Abstract
An effort has been made to isolate individual catechin compounds from green tea leaves in their pure form by electrophoresis. In the present study total polyphenol extraction was carried out initially and estimated through spectrophotometric and HPLC methods. Extracted polyphenol was separated on 0.7% agarose gel and visualized at 360 nm. Fragmented individual compounds were gel eluted with methanol and confirmed as (-)-epigallocatechin (EGC), (-)-epicatechin (EC), (-)-epicatechin gallate (ECG), and (-)-epigallocatechin gallate (EGCG) by HPLC. The method developed describes a suitable method for the isolation of valuable molecules in tea.
Author information
Author/s: John, K M Mariya (KM); Vijayan, Deepu (D); Rahul, P R (PR); Joshi, S D (SD); Kumar, R Raj (RR); Mandal, A K A (AK);
Affiliation: Division of Plant Physiology, UPASI Tea Research Institute, Nirar Dam BPO, Valparai, 642 127 TN, India. johnupasi(-atsign-)rediffmail.com
Journal and publication information
Publication Type: Journal Article
Journal: Journal of agricultural and food chemistry (J Agric Food Chem), published in United States. (Language: eng)
Reference: 2006-Apr; vol 54 (issue 8) : pp 2828-31
Dates: Created 2006/04/12; Completed 2006/06/20;
PMID: 16608196, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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