Lipoic acid inhibits expression of ICAM-1 and VCAM-1 by CNS endothelial cells and T cell migration into the spinal cord in experimental autoimmune encephalomyelitis.

Full Abstract

Lipoic acid (LA) suppresses and treats murine experimental autoimmune encephalomyelitis (EAE), which models multiple sclerosis. However, the mechanisms by which LA mediates its effects in EAE are only partially known. In the present study, LA (25, 50 and 100 microg/ml) inhibited upregulation of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in tumor necrosis factor-alpha (TNF-alpha) stimulated cultured brain endothelial cells. Immunohistochemical analysis of spinal cords from SJL mice that had received LA (100 mg/kg/day) following immunization to induce EAE exhibited markedly reduced expression of ICAM-1 and VCAM-1 compared with that of EAE mice receiving saline. Co-localization analysis showed that ICAM-1 and VCAM-1 expression increased over endothelial cells (staining positive for von Willebrand factor, vWF) in EAE and that LA decreased the expression levels to that observed in naïve mice. Spinal cords from mice receiving LA had significantly reduced inflammation (decreased CD4 and CD11b staining) as compared to EAE mice that received saline. Overall, our data suggest that the anti-inflammatory effects of LA in EAE may be partly due to inhibition of ICAM-1 and VCAM-1 expression by central nervous system (CNS) endothelial cells.

Learn Faster Today Improve your study skills

Author information

Author/s: Chaudhary, Priya (P); Marracci, Gail H (GH); Bourdette, Dennis N (DN);

Affiliation: Department of Neurology, L226, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, 97239, USA. chaudhare(-atsign-)ohsu.edu

Grants: AT P50 AT00066-01 (Agency:NCCAM NIH HHS)

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: Journal of neuroimmunology (J Neuroimmunol), published in Netherlands. (Language: eng)

Reference: 2006-Jun; vol 175 (issue 1-2) : pp 87-96

Dates: Created 2006/05/29; Completed 2006/07/26; Revised 2007/11/14;

PMID: 16644024, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Cell Migration Inhibition
Down-Regulation - drug effects
Encephalomyelitis, Autoimmune, Experimental - drug therapy; immunology; metabolism; pathology
Endothelial Cells - drug effects; metabolism
Gene Expression Regulation - drug effects; physiology

Intercellular Adhesion Molecule-1 - biosynthesis
Mice
Spinal Cord - drug effects; metabolism; pathology
T-Lymphocytes - cytology; drug effects; metabolism
Thioctic Acid - pharmacology; therapeutic use
Vascular Cell Adhesion Molecule-1 - biosynthesis

Associated Chemicals: Vascular Cell Adhesion Molecule-1 (0) ; Intercellular Adhesion Molecule-1 (126547-89-5) ; Thioctic Acid (62-46-4)

These are the highest related articles currently in the database:

See 100+ related articles.