IPSE/alpha-1, a major secretory glycoprotein antigen from schistosome eggs, expresses the Lewis X motif on core-difucosylated N-glycans.

Full Abstract

Schistosomes are parasitic flatworms that infect millions of people in (sub)tropical areas around the world. Glycoconjugates of schistosomes play a critical role in the interaction of the different developmental stages of the parasite with the host. In particular, glycosylated components of the eggs produced by the adult worm pairs living in the bloodstream are strongly immunogenic. We have investigated the glycosylation of interleukin-4-inducing factor from schistosome eggs (IPSE/alpha-1), a major secretory egg antigen from Schistosoma mansoni that triggers interleukin-4 production in human basophils, by MS analysis of tryptic glycopeptides. Nanoscale LC-MS(/MS) and MALDI-TOF(/TOF)-MS studies combined with enzymatic degradations showed that monomeric IPSE/alpha-1 contains two N-glycosylation sites, which are each occupied for a large proportion with core-difucosylated diantennary glycans that carry one or more Lewis X motifs. Lewis X has been reported as a major immunogenic glycan element of schistosomes. This is the first report both on the expression of Lewis X on a specific schistosome egg protein and on a protein-specific glycosylation analysis of schistosome eggs.

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Author information

Author/s: Wuhrer, Manfred (M); Balog, Crina I A (CI); Catalina, M I (MI); Jones, Frances M (FM); Schramm, Gabriele (G); Haas, Helmut (H); Doenhoff, Michael J (MJ); Dunne, David W (DW); Deelder, André M (AM); Hokke, Cornelis H (CH);

Affiliation: Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands. m.wuhrer(-atsign-)lumc.nl

Journal and publication information

Publication Type: Journal Article

Journal: The FEBS journal (FEBS J), published in England. (Language: eng)

Reference: 2006-May; vol 273 (issue 10) : pp 2276-92

Dates: Created 2006/05/02; Completed 2006/07/13;

PMID: 16650003, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Sequence
Animals
Antigens, Helminth - chemistry; metabolism
Carbohydrate Conformation
Carbohydrate Sequence
Egg Proteins - chemistry; genetics; metabolism
Fucose - chemistry; metabolism

Glycoproteins - chemistry; metabolism
Helminth Proteins - chemistry; genetics; metabolism
Molecular Sequence Data
Ovum - immunology
Polysaccharides - chemistry; metabolism
Schistosoma mansoni - immunology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Associated Chemicals: Antigens, Helminth (0) ; Egg Proteins (0) ; Glycoproteins (0) ; Helminth Proteins (0) ; IPSE protein, Schistosoma mansoni (0) ; Polysaccharides (0) ; Fucose (3713-31-3)

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