Extract EGb 761 pretreatment limits ubiquitin positive aggregates in rabbit spinal cord neurons after ischemia-reperfusion.

Full Abstract

1. Ubiquitin immunohistochemistry was used for investigation of time dependent changes of ubiquitin in the nerve cells reacting to ischemic/reperfusion damage. In the rabbit spinal cord ischemia model a period of 30 min ischemia followed by 24 and 72 h of reperfusion caused neuronal degeneration selectively in the ventral horn motor neurons as well as interneurons of the intermediate zone. 2. Ubiquitin aggregates were accumulated in the neurons of lamina IX and the neurons of intermediate zone destined to die 72 h after 30 min of the spinal cord ischemia. 3. The activation of ubiquitin hydrolytic system is related to a defective homeostasis and could trigger different degenerative processes. Having in mind this, we used EGb 761 to rescue the motor neurons and interneurons against ischemia/reperfusion damage. Our results show that after 30 min of ischemia and 24 or 72 h of reperfusion with EGb 761 pre-treatment for 7 days the vulnerable neurons in the intermediate zone and lamina IX exhibit marked elevation of ubiquitin-positive granules in the cytoplasm, dendrites and nuclei. Abnormal protein aggregates have not been observed in these cells. 4. The rabbits were completely paraplegic after 30 min of ischemia and 24 or 72 h of reperfusion. However, after 7 days EGb 761 pre-treatment, 30 min of ischemia and 24 or 72 h of reperfusion the animals did not show paraplegia. 5. Evaluated ubiquitin-positive neurons of the L(5)-L(6) segments showed significant decrease in number and significant increase of density after 30 min of ischemia followed by 24 h and mainly 72 h of reperfusion. Ubiquitin immunohistochemistry confirmed the protective effect of EGb 761 against ischemia/reperfusion damage in the rabbit spinal cord.

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Author information

Author/s: Mechírová, Eva (E); Feriková, Marianna (M); Domoráková, Iveta (I);

Affiliation: Department of Histology and Embryology, Faculty of Medicine, P. J. Safárik University, Kosice, Slovak Republic. mechir(-atsign-)post.sk

Journal and publication information

Publication Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: Cellular and molecular neurobiology (Cell Mol Neurobiol), published in United States. (Language: eng)

Reference: -2006 Oct-Nov; vol 26 (issue 7-8) : pp 1443-52

Dates: Created 2006/12/07; Completed 2007/05/24;

PMID: 16670948, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Cell Aggregation - drug effects
Ginkgo biloba
Male
Neuroprotective Agents - therapeutic use
Paraplegia - prevention & control
Plant Extracts - therapeutic use

Plant Extracts - therapeutic use
Premedication
Rabbits
Reperfusion Injury - prevention & control
Spinal Cord - drug effects; metabolism
Spinal Cord Ischemia - metabolism; pathology; prevention & control
Ubiquitin - metabolism

Associated Chemicals: Ginkgo biloba extract 761 (0) ; Neuroprotective Agents (0) ; Plant Extracts (0) ; Ubiquitin (0)

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