Comparison of two whole genome amplification methods for STR genotyping of LCN and degraded DNA samples.

Full Abstract

The analysis of LCN or highly degraded DNA samples presents a challenge for forensic science. Improving the quantity and/or quality of samples would greatly increase the profiling success rate from LCN and degraded samples. Whole genome amplification (WGA) is one method that has such potential. Two commercially available WGA kits, GenomePlex and GenomiPhi, were investigated for use on LCN and degraded DNA samples. Both kits amplified genomic DNA, producing microgram quantities from sub-nanogram templates. Profiling success of LCN DNA samples was increased, with improvements of over 700% from 10pg template DNA compared to non-WGA-amplified control samples. The amplification success with degraded DNA was also improved by WGA. Degraded DNA was simulated using restriction enzymes to demonstrate that the application of WGA can result in the typing of STR loci that could not previously be amplified. An increase in artefacts, such as stutter alleles and amplification biases, were observed in many samples. Results show that WGA is capable of increasing both the quality and quantity of DNA, and has the potential to improve profiling success from difficult samples in forensic casework.

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Author information

Author/s: Ballantyne, Kaye N (KN); van Oorschot, Roland A H (RA); Mitchell, R John (RJ);

Affiliation: Victoria Police Forensic Services Department, Macleod, VIC, Australia. kaye.ballantyne(-atsign-)police.vic.gov.au

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Forensic science international (Forensic Sci Int), published in Ireland. (Language: eng)

Reference: 2007-Feb; vol 166 (issue 1) : pp 35-41

Dates: Created 2007/02/02; Completed 2007/04/03;

PMID: 16687226, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

DNA - analysis
DNA Fingerprinting - methods
Forensic Pathology
Genome

Genome
Humans
Microsatellite Repeats
Nucleic Acid Amplification Techniques - methods

Associated Chemicals: DNA (9007-49-2)

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