A paramagnetic contrast agent with myeloperoxidase-sensing properties.

Full Abstract

Myeloperoxidase (MPO) is an essential component of inflammatory response in norm and pathology. With an ultimate goal of non-invasive imaging of MPO we used a gadolinium-chelating bis(5-hydroxytrytamide) derivative of diethylenetetraamine pentaacetic acid (L1-Gd3+ salt) as a paramagnetic sensor of enzymatic activity. We tested whether L1-Gd3+ is active in reducing the oxidized form of myeloperoxidase, generated as a result of hydrogen peroxide reduction by the enzyme. We expected that upon activation by MPO/H2O2 L1-Gd3+ would not only oligomerise but also bind to other macromolecules present in the media and that the overall process will give rise to a net T1-weighted MRI signal increase.

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Author information

Author/s: Querol, Manel (M); Chen, John W (JW); Bogdanov, Alexei A (AA);

Affiliation: University of Massachusetts Medical School, 55 Lake Avenue, Worcester, Massachusetts 01655, USA.

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: Organic & biomolecular chemistry (Org Biomol Chem), published in England. (Language: eng)

Reference: 2006-May; vol 4 (issue 10) : pp 1887-95

Dates: Created 2006/05/11; Completed 2007/08/06;

PMID: 16688334, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Contrast Media - chemistry
Gadolinium - chemistry
Gadolinium DTPA
Hydrogen Peroxide - metabolism

Hydrogen Peroxide - metabolism
Ligands
Magnetic Resonance Imaging - methods
Peroxidase - metabolism

Associated Chemicals: Contrast Media (0) ; Ligands (0) ; Gadolinium (7440-54-2) ; Hydrogen Peroxide (7722-84-1) ; Gadolinium DTPA (80529-93-7) ; Peroxidase (EC 1.11.1.7)

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