Identification of nuclear spliceosomal antigens targeted by NOD mouse antibodies following sodium iodide intake.

Full Abstract

The non-obese diabetic (NOD) mouse spontaneously develops a range of autoreactive responses including an autoantibody response to nuclear antigens. As elevated dietary iodine has been shown to increase thyroid autoimmune pathology in NOD mice, the effect of sodium iodide (NaI) on the development of anti-nuclear antibodies (ANA) was assessed. Interestingly, the NaI symporter is expressed in both thyroid and salivary glands. Elevated dietary iodine was found to increase the percentage of male NOD mice developing autoantibodies. Specifically, the nuclear autoantibodies that develop in NOD mice were shown to target specific spliceosomal components. The target specificity of the autoantibodies was determined using recombinant spliceosomal proteins and shown to include U1A, U170K, U2B'', U2A', as well as the Sm proteins D1, D2, and B. The autoantibody isotypes most consistently represented were IgG2a and IgG2b.

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Author information

Author/s: Thompson, C (C); Pomeranz Krummel, D A (DA); Jacobsen, H (H); Nagai, K (K); Cooke, A (A);

Affiliation: University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge, CB2 1QP, UK.

Grants: (Agency:Wellcome Trust)

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Autoimmunity (Autoimmunity), published in England. (Language: eng)

Reference: 2006-Mar; vol 39 (issue 2) : pp 99-106

Dates: Created 2006/05/15; Completed 2006/07/31; Revised 2008/11/21;

PMID: 16698665, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Sequence
Animals
Antibodies, Antinuclear - biosynthesis; blood
Antibody Specificity
Autoantigens - genetics; immunology; isolation & purification
Blotting, Western
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry

Male
Mice
Mice, Inbred NOD
Molecular Sequence Data
RNA-Binding Proteins - genetics; immunology
Ribonucleoprotein, U1 Small Nuclear - genetics; immunology
Ribonucleoproteins, Small Nuclear - genetics; immunology
Sjogren's Syndrome - etiology; immunology
Sodium Iodide - administration & dosage; toxicity
Spliceosomes - immunology
snRNP Core Proteins

Associated Chemicals: Antibodies, Antinuclear (0) ; Autoantigens (0) ; RNA-Binding Proteins (0) ; Ribonucleoprotein, U1 Small Nuclear (0) ; Ribonucleoproteins, Small Nuclear (0) ; SNRPB2 protein, human (0) ; U1A protein (0) ; snRNP Core Proteins (0) ; Sodium Iodide (7681-82-5)

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