Research article summary (published 30 May 2006):

Ultrasound stimulation of types I and III collagen expression of tendon cell and upregulation of transforming growth factor beta.

Full Abstract

Traumatic tendon injuries are commonly treated with ultrasound. However, previous research has not examined the molecular mechanism of this therapeutic effect on collagen synthesis of tendon cells. This study was designed to determine the effect of ultrasound on the expression of type I and type III collagen of tendon cells intrinsic to rat Achilles tendon. Whether a correlation exits between this effect and the expression of transforming growth factor beta (TGF-beta), which enhances collagen synthesis, was also investigated. Tendon cells after ultrasound treatment and protein expression of types I and III collagen were determined by immunocytochemistry. The mRNA expressions of alpha1(I) procollagen, alpha1(III) procollagen, and TGF-beta were determined by reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, the concentration of TGF-beta in conditioned medium was evaluated by enzyme-linked immunosorbent assay (ELISA). Immunocytochemical staining revealed that ultrasound-treated tendon cells were stained more strongly for types I and III collagen than were control cells. Upregulation of procollagen alpha1(I) gene, procollagen alpha1(III) gene, and TGF-beta at the mRNA level was confirmed by RT-PCR. A dose-dependent increase in the concentration of TGF-beta in conditioned medium obtained from cells treated with ultrasound was demonstrated by ELISA assay (p = 0.043). In conclusion, ultrasound stimulates the expression of type I and type III collagen in a process that is likely mediated by the upregulation of TGF-beta. (c) 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Author information

Author/s: Tsai, Wen-Chung (WC); Pang, Jong-Hwei S (JH); Hsu, Chih-Chin (CC); Chu, Ngok-Kiu (NK); Lin, Miao-Sui (MS); Hu, Ching-Fang (CF);

Affiliation: Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, 5, Fu-Shin Street, Kweishan, Taoyuan 333, Taiwan. tsaiwc(-atsign-)adm.cgmh.org.tw

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (J Orthop Res), published in United States. (Language: eng)

Reference: 2006-Jun; vol 24 (issue 6) : pp 1310-6

Dates: Created 2006/05/22; Completed 2006/06/29; Revised 2006/11/15;

PMID: 16705693, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Achilles Tendon - injuries; metabolism; pathology Animals Collagen Type I - biosynthesis; genetics; metabolism Collagen Type III - biosynthesis; genetics Enzyme-Linked Immunosorbent Assay Immunohistochemistry Protein Biosynthesis RNA, Messenger - metabolism

RNA, Messenger - metabolism Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Tendon Injuries - therapy Transforming Growth Factor beta - genetics; metabolism Ultrasonic Therapy Up-Regulation

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; RNA, Messenger (0) ; Transforming Growth Factor beta (0) ; collagen type I, alpha 1 chain (0)

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