Research article summary (published 30 May 2006):

CXCR3 and its ligands in a murine model of obliterative bronchiolitis: regulation and function.

Full Abstract

Lung transplantation remains the only effective therapy for patients with end-stage lung disease, but survival is limited by the development of obliterative bronchiolitis (OB). The chemokine receptor CXCR3 and two of its ligands, CXCL9 and CXCL10, have been identified as important mediators of OB. However, the relative contribution of CXCL9 and CXCL10 to the development of OB and the mechanism of regulation of these chemokines has not been well defined. In this study, we demonstrate that CXCL9 and CXCL10 are up-regulated in unique patterns following tracheal transplantation in mice. In these experiments, CXCL9 expression peaked 7 days posttransplant, while CXCL10 expression peaked at 1 day and then again 7 days posttransplant. Expression of CXCL10 was also up-regulated in a novel murine model of lung ischemia, and in bronchoalveolar lavage fluid taken from human lungs 24 h after lung transplantation. In further analysis, we found that 3 h after transplantation CXCL10 is donor tissue derived and not dependent on IFN-gamma or STAT1, while 24 h after transplantation CXCL10 is from recipient tissue and regulated by IFN-gamma and STAT1. Expression of both CXCL9 and CXCL10 7 days posttransplant is regulated by IFN-gamma and STAT1. Finally, we demonstrate that deletion of CXCR3 in recipients reduces airway obliteration. However, deletion of either CXCL9 or CXCL10 did not affect airway obliteration. These data show that in this murine model of obliterative bronchiolitis, these chemokines are differentially regulated following transplantation, and that deletion of either chemokine alone does not affect the development of airway obliteration.

Author information

Author/s: Medoff, Benjamin D (BD); Wain, John C (JC); Seung, Edward (E); Jackobek, Ryan (R); Means, Terry K (TK); Ginns, Leo C (LC); Farber, Joshua M (JM); Luster, Andrew D (AD);

Affiliation: Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA.

Grants: K08 HL072775 (Agency:NHLBI NIH HHS) ; R01 AI050892 (Agency:NIAID NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol), published in United States. (Language: eng)

Reference: 2006-Jun; vol 176 (issue 11) : pp 7087-95

Dates: Created 2006/05/19; Completed 2006/07/13; Revised 2008/11/21;

PMID: 16709871, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Animals Bronchiolitis Obliterans - genetics; immunology; metabolism; therapy Cell Migration Inhibition Chemokine CXCL10 Chemokine CXCL9 Chemokines, CXC - biosynthesis; deficiency; genetics; physiology Disease Models, Animal Gene Deletion Humans Interferon-gamma - physiology Ligands Lung - blood supply; immunology; metabolism

Lymphocytes - cytology; immunology; metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Pulmonary Fibrosis - genetics; immunology; metabolism; prevention & control Receptors, CXCR3 Receptors, Chemokine - biosynthesis; deficiency; genetics; physiology Reperfusion Injury - immunology; metabolism STAT1 Transcription Factor - physiology Trachea - immunology; metabolism; transplantation Up-Regulation - genetics; immunology

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: CXCR3 protein, human (0) ; Chemokine CXCL10 (0) ; Chemokine CXCL9 (0) ; Chemokines, CXC (0) ; Cxcl9 protein, mouse (0) ; Cxcr3 protein, mouse (0) ; Ligands (0) ; Receptors, CXCR3 (0) ; Receptors, Chemokine (0) ; STAT1 Transcription Factor (0) ; Interferon-gamma (82115-62-6)

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