Expression of calcitonin receptor in rat mammary gland during lactation.

Full Abstract

Calcitonin (CT) and calcitonin receptor (CTR) have been reported to play an important role in mammary tissue during pregnancy, lactation, and involution. In the present study, the expression and distribution of CTR mRNA in rat mammary tissue during pregnancy and lactation were investigated. As measured by real-time RT-PCR, CTR mRNA levels were increased only slightly during pregnancy, but increased markedly immediately postpartum and remained elevated through lactation, with the highest levels observed 14 days postpartum. In situ hybridization analysis showed that intense CTR mRNA signals were detected in the whole mammary gland. We performed immunohistochemistry to determine distribution of CTR in the mammary epithelium. CTR has been reported to act as an amylin receptor when heterodimerized with receptor activity modifying protein-1 (RAMP1) or RAMP3. mRNA expression of RAMP1 and RAMP3 in mammary tissue decreased during pregnancy and lactation, and amylin mRNA was undetectable, suggesting that up-regulated CTR in lactating mammary tissues binds CT rather than amylin. In primary cultures of mammary cells isolated from rat dams 14 days postpartum, CT produced a statistically significant decrease in thymidine incorporation. These results suggest that up-regulation of CTR during lactation may contribute to inhibition of mammary epithelial cell proliferation.

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Author information

Author/s: Ishii, Aiko (A); Nakamura, Misa (M); Nakamura, Atsushi (A); Takeda, Koichi (K); Han, Bo (B); Kakudo, Kennichi (K);

Affiliation: Department of Pathology, Wakayama Medical University, Wakayama, Japan.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Endocrine journal (Endocr J), published in Japan. (Language: eng)

Reference: 2006-Jun; vol 53 (issue 3) : pp 317-24

Dates: Created 2006/06/27; Completed 2006/10/20; Revised 2006/11/15;

PMID: 16710074, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Calcitonin - metabolism; pharmacology
DNA - biosynthesis; drug effects
Female
Gene Expression Profiling
Immunohistochemistry
In Situ Hybridization
Intracellular Signaling Peptides and Proteins - metabolism
Lactation - metabolism
Male

Mammary Glands, Animal - metabolism
Membrane Proteins - metabolism
Pregnancy
RNA, Messenger - metabolism
Rats
Rats, Inbred WF
Receptors, Calcitonin - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tissue Distribution
Weaning

Associated Chemicals: Intracellular Signaling Peptides and Proteins (0) ; Membrane Proteins (0) ; RNA, Messenger (0) ; Receptors, Calcitonin (0) ; receptor-activity-modifying protein (0) ; Calcitonin (9007-12-9) ; DNA (9007-49-2)

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