Research article summary (published May 2006):

Presynaptic serotonergic modulation of 5-HT and acetylcholine release in the hippocampus and the cortex of 5-HT1B-receptor knockout mice.

Full Abstract

Lesioning of serotonergic afferents increases hippocampal ACh release and attenuates memory deficits produced by cholinergic lesions. Improved memory performance described in 5-HT1B-knockout (KO) mice might thus be due to a weaker 5-HT1B-mediated inhibitory influence of 5-HT on hippocampal ACh release. The selective delay-dependent impairment of working memory observed in these KO mice suggests, however, that cortical regions also participate in task performance, possibly via indirect influences of 5-HT on ACh release. To provide neuropharmacological support for these hypotheses we measured evoked ACh and 5-HT release in hippocampal and cortical slices of wild-type (WT) and 5-HT1B KO mice. Superfused slices (preincubated with [3H]choline or [3H]5-HT) were electrically stimulated in the absence or presence of 5-HT1B receptor ligands. In hippocampus and cortex, 5-HT1B agonists decreased and antagonists increased 5-HT release in WT, but not in 5-HT1B KO mice. In 5-HT1B KO mice, 5-HT release was enhanced in both structures, while ACh release (in nCi) was reduced. ACh release was inhibited by 5-HT1B agonists in hippocampal (not cortical) slices of WT but not of 5-HT1B KO mice. Our data (i) confirm the absence of autoinhibition of 5-HT release in 5-HT1B-KO mice, (ii) demonstrate a reduced release of ACh, and the absence of 5-HT1B-receptor-mediated inhibition of ACh release, in the hippocampus and cortex of 5-HT1B-KO mice, and (iii) are compatible with an indirect role of cortical ACh in the working memory impairment observed in these KO mice.

Learn Faster Today      Improve your study skills

Author information

Author/s: Rutz, Susanne (S); Riegert, Céline (C); Rothmaier, Anna Katharina (AK); Buhot, Marie-Christine (MC); Cassel, Jean-Christophe (JC); Jackisch, Rolf (R);

Affiliation: Institute of Experimental and Clinical Pharmacology and Toxicology, Laboratory of Neuropharmacology, University of Freiburg, Hansastrasse 9A, D-79104 Freiburg, Germany.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Brain research bulletin (Brain Res Bull), published in United States. (Language: eng)

Reference: 2006-Jun; vol 70 (issue 1) : pp 81-93

Dates: Created 2006/06/05; Completed 2006/09/05; Revised 2006/11/15;

PMID: 16750486, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acetylcholine - metabolism Analysis of Variance Animals Cerebral Cortex - cytology; drug effects; radiation effects Choline - metabolism Dose-Response Relationship, Drug Electric Stimulation - methods Hippocampus - cytology; drug effects; radiation effects Male Mice

Mice, Knockout Presynaptic Terminals - drug effects; metabolism; radiation effects Pyridines - pharmacology Pyrroles - pharmacology Quipazine - analogs & derivatives; pharmacology Receptor, Serotonin, 5-HT1B - deficiency Serotonin - metabolism Serotonin Agonists - pharmacology Serotonin Antagonists - pharmacology Tritium - metabolism

Associated Chemicals: Pyridines (0) ; Pyrroles (0) ; Receptor, Serotonin, 5-HT1B (0) ; Serotonin Agonists (0) ; Serotonin Antagonists (0) ; Tritium (10028-17-8) ; 3-(1,2,5,6-tetrahydropyrid-4-yl)pyrrolo(3,2-b)pyrid-5-one (127792-75-0) ; Quipazine (4774-24-7) ; Serotonin (50-67-9) ; Acetylcholine (51-84-3) ; Choline (62-49-7) ; 6-nitroquipazine (77372-73-7)

Related articles

These are the highest related articles currently in the database:

• 5-HT1B receptor-mediated presynaptic inhibition of GABA release in the suprachiasmatic nucleus. 14 Feb 2005
• Reduced hypophagic effects of d-fenfluramine and the 5-HT2C receptor agonist mCPP in 5-HT1B receptor knockout mice. 6 May 2004
• 5-HT1B receptors modulate the feeding inhibitory effects of enterostatin. 24 Oct 2005
• [Mechanism of action of antidepressant drugs: importance of genetically modified mice in the pharmacological in vivo approach] 30 Aug 2005
• Impulsive-like behavior in differential-reinforcement-of-low-rate 36 s responding in mice depends on training history. 7 Jan 2004
• Sucrose intake and fasting glucose levels in 5-HT(1A) and 5-HT(1B) receptor mutant mice. 7 Nov 2007
• Lack of serotonin1B receptor expression leads to age-related motor dysfunction, early onset of brain molecular aging and reduced longevity. 8 Apr 2007
• Juvenile 5HT(1B) receptor knockout mice exhibit reduced pharmacological sensitivity to 5HT(1A) receptor activation. 30 Jul 2004
• Light-induced Fos expression is attenuated in the suprachiasmatic nucleus of serotonin 1B receptor knockout mice. 9 Apr 2006

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.