Mapping of rat hippocampal neurons with NeuN after ischemia/reperfusion and Ginkgo biloba extract (EGb 761) pretreatment.

Full Abstract

1. The neuroprotective effect of Ginkgo biloba extract (EGb 761) against transient forebrain ischemia following 7 days of reperfusion was studied in male Wistar rats after four-vessel occlusion for 20 min. 2. NeuN, a neuronal specific nuclear protein was used for immunohistochemical detection of surviving pyramidal neurons in the hippocampus, as well as counterstaining with hematoxylin in the same sections for detection of neurons that underwent delayed neuronal death and for glial nuclei staining. GFAP immunohistochemistry was used for detection of astrocytes in the studied area of CA1 region. 3. In the group of rats pretreated 7 days with Ginkgo biloba extract (EGb 761), following 20 min of ischemia and 7 days of reperfusion without EGb 761, increased number of NeuN immunoreactive cells were counted in the most vulnerable CA1 pyramidal layer of hippocampus. On the other hand, the group of rats with 7 days of EGb 761 pretreatment following 20 min of ischemia and 7 days of reperfusion with EGb 761 showed decreased number of surviving NeuN immunoreactive CA1 pyramidal cells in comparison with the first above-mentioned experimental group. 4. Increased number of reactive astrocytes immunolabeled for GFAP (Glial fibrilary acidic protein) was observed in both experimental groups in the stratum oriens and stratum lacunosum and moleculare. 5. Twenty minutes of ischemia is lethal for most population of CA1 pyramidal cell layer. Our results showed that prophylactic oral administration of Ginkgo biloba extract (EGb 761) in the dose 40 mg/kg/day during the 7 days protects the most vulnerable CA1 pyramidal cells against 20 min of ischemia.

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Author information

Author/s: Domoráková, Iveta (I); Burda, Jozef (J); Mechírová, Eva (E); Feriková, Marianna (M);

Affiliation: Department of Histology and Embryology, Faculty of Medicine, P. J. Safárik University, Kosice, Slovak Republic. domorak(-atsign-)upjs.sk

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: Cellular and molecular neurobiology (Cell Mol Neurobiol), published in United States. (Language: eng)

Reference: -2006 Oct-Nov; vol 26 (issue 7-8) : pp 1193-204

Dates: Created 2006/12/07; Completed 2007/05/24;

PMID: 16758319, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Astrocytes - drug effects; metabolism
Brain Mapping
Ginkgo biloba
Hippocampus - drug effects; metabolism; pathology
Immunohistochemistry
Male
Nerve Tissue Proteins - metabolism

Nerve Tissue Proteins - metabolism
Neuroprotective Agents - therapeutic use
Nuclear Proteins - metabolism
Plant Extracts - therapeutic use
Pyramidal Cells - drug effects; metabolism
Rats
Rats, Wistar
Reperfusion Injury - metabolism; pathology; prevention & control

Associated Chemicals: Ginkgo biloba extract 761 (0) ; Nerve Tissue Proteins (0) ; Neuroprotective Agents (0) ; Nuclear Proteins (0) ; Plant Extracts (0)

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