Research article summary (published 6 Jun 2006):

The regression of a canine Langerhans cell tumour is associated with increased expression of IL-2, TNF-alpha, IFN-gamma and iNOS mRNA.

Full Abstract

Canine cutaneous histiocytoma is a benign epidermal neoplasm of Langerhans cell origin, which usually displays spontaneous regression. Based on the degree of lymphocytic infiltration, 30 histiocytomas were classified into four groups representing different stages of tumour regression. To elucidate further the mechanisms of the antitumour immune response CD3+, CD21+, CD4+, CD8+ and myeloid/histiocyte antigen+ inflammatory cells were differentiated by immunohistochemistry and quantified. In addition, the number of apoptotic cells was detected using the TdT-mediated biotin-dUTP nick-end labelling (TUNEL) method. Furthermore, the expression of interleukin- (IL-2), IL-12(p40), tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), IL-10 and transforming growth factor-beta (TGF-beta) as well as inducible nitric oxid synthase (iNOS) mRNA was determined by reverse transcription-polymerase chain reaction (RT-PCR). Phenotyping of inflammatory cells revealed a significantly increased infiltration of all lymphocyte subsets and myeloid/histiocytic cells with the onset of tumour regression. The latter was significantly correlated to up-regulation of IL-2, TNF-alpha, IFN-gamma and iNOS mRNA expression. Expression of remaining cytokines and percentage of apoptotic cells showed no group-specific changes. The results indicate an initial infiltration of CD4+ T cells followed by increased expression of Th1 cytokines and recruitment of antitumour effector cells as the principal mechanism for tumour regression. Canine cutaneous histiocytoma is a unique example for an effective immune response in a naturally occurring neoplasm derived from epidermal Langerhans cells and might represent a valuable animal model to investigate tumour immunity.

Learn Faster Today      Improve your study skills

Author information

Author/s: Kaim, Ute (U); Moritz, Andreas (A); Failing, Klaus (K); Baumgärtner, Wolfgang (W);

Affiliation: Department of Pathology, University of Veterinary Medicine, Hannover, Germany.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Immunology (Immunology), published in England. (Language: eng)

Reference: 2006-Aug; vol 118 (issue 4) : pp 472-82

Dates: Created 2006/08/09; Completed 2006/09/21; Revised 2008/11/21;

PMID: 16764690, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Apoptosis Biological Markers Cell Count Cytokines - genetics Dog Diseases - immunology; pathology Dogs Female Gene Expression Regulation, Neoplastic Histiocytoma, Benign Fibrous - immunology; pathology; veterinary In Situ Nick-End Labeling Interferon-gamma - genetics

Interferon-gamma - genetics Interleukin-2 - genetics Langerhans Cells - immunology; pathology Macrophages - immunology; pathology Male Neoplasm Regression, Spontaneous - immunology; pathology Nitric Oxide Synthase Type II - genetics RNA, Messenger - analysis Reverse Transcriptase Polymerase Chain Reaction Skin Neoplasms - immunology; pathology; veterinary Statistics, Nonparametric Tumor Necrosis Factor-alpha - genetics

Associated Chemicals: Biological Markers (0) ; Cytokines (0) ; Interleukin-2 (0) ; RNA, Messenger (0) ; Tumor Necrosis Factor-alpha (0) ; Interferon-gamma (82115-62-6) ; Nitric Oxide Synthase Type II (EC 1.14.13.39)

Related articles

These are the highest related articles currently in the database:

• Morphologic and biologic characteristics of the canine cutaneous histiocytoma. 30 Dec 1968
• Expression of major histocompatibility complex class II antigen in neoplastic cells of canine cutaneous histiocytoma. 16 Mar 1998
• E-cadherin expression in canine cutaneous histiocytomas. 17 Apr 2008
• Hereditary multifocal renal cystadenocarcinomas and nodular dermatofibrosis in 51 German shepherd dogs. 30 Oct 1997
• Activated macrophages migrate to the subcutaneous tumor site via the peritoneum: a novel route of cell trafficking. 13 May 2001
• Apoptosis and mitosis in tumours of the skin and subcutaneous tissues of the dog. 30 Mar 1999
• Cytokine profiles in spontaneously regressing basal cell carcinomas. 29 Jun 2000
• Histopathological survey of neoplasms in flat-coated retrievers, 1990 to 1998. 7 Sep 2000
• A mutation in the canine BHD gene is associated with hereditary multifocal renal cystadenocarcinoma and nodular dermatofibrosis in the German Shepherd dog. 5 Oct 2003

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.