Can cryoglobulins interfere with the measurement of IgM antiphosphatidylethanolamine antibodies by ELISA?

Full Abstract

Clinical manifestations of the antiphospholipid antibody syndrome (APS) have been recently related to the presence of phosphatidylethanolamine antibodies (aPE). However, it is well known that some molecules such as cryoglobulins, immunoglobulins that undergo a reversible precipitation at low temperatures, may interfere with biological assays. With this in view, we report the case of a patient with APS who was positive for both IgM aPE and type III cryoglobulinemia. Moreover, we show for this patient a potential implication of aPE in the cryoprecipitate formation. To further analyze the potential association between cryoglobulins and aPE, and also the possible consequences for aPE assay, we selected 55 patients according to positivity for both IgM aPE and cryoglobulinemia. Determination of IgM aPE levels was made before and after removal of cryoprecipitate from the serum. Of the 55 selected patients, 52 (95%) presented no significant difference for IgM aPE levels before and after cryoprecipitation. These results were ascertained whatever the aPE levels and clinical status of the patient. Taken together, our results indicate that cryoprecipitation does not interfere in most cases (95%) with the dosage of IgM aPE. Thus, IgM aPE do not appear to be involved in the formation of the cryoprecipitate.

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Author information

Author/s: Bardin, N (N); Pommier, G (G); Sanmarco, M (M);

Affiliation: Fédération Autoimmunité et Thrombose, Hôpital de la Conception, Marseille, France. nathalie.bardin(-atsign-)mail.ap-hm.fr

Journal and publication information

Publication Type: Case Reports; Comparative Study; Journal Article

Journal: Thrombosis research (Thromb Res), published in United States. (Language: eng)

Reference: 2007-; vol 119 (issue 4) : pp 441-6

Dates: Created 2007/03/19; Completed 2007/04/23;

PMID: 16766022, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult
Antibodies, Antiphospholipid - analysis
Anticoagulants - therapeutic use
Antiphospholipid Syndrome - diagnosis; drug therapy
Cohort Studies
Cryoglobulins - analysis; classification
Enzyme-Linked Immunosorbent Assay - methods

Enzyme-Linked Immunosorbent Assay - methods
Female
Humans
Immunoglobulin M - blood; immunology
Male
Phenindione - analogs & derivatives; therapeutic use
Treatment Outcome

Associated Chemicals: Antibodies, Antiphospholipid (0) ; Anticoagulants (0) ; Cryoglobulins (0) ; Immunoglobulin M (0) ; Phenindione (83-12-5) ; fluindione (957-56-2)

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