[In vivo fluorescent microscopy of the liver microcirculation after embolization with a newly developed trans-proper hepatic arterial infusion animal model]

Full Abstract

OBJECTIVE:
To evaluate the value of the in vivo fluorescent microscopy in studying the changes of liver microcirculation after embolization with a newly developed animal model for tans-proper hepatic arterial infusion, and to summarize the method of making this animal model.

METHODS:
Ten Sprague-Dawley rats were used in this study. After a midline abdominal incision, microcatheter was placed into the gastroduodenal artery (GDA). The tip of the catheter was placed facing the orifice of proper hepatic artery. After infusions of 0.02%, 0.1%, 0.5%, 1% fluorescent sodium, fluorescent microscopy was used to evaluate the liver microcirculation. The image quality was then accessed. Embolization was obtained by injections of Lipiodol and Degradable Starch Microspheres (DSM) from the microcatheter. Corresponding changes of the liver microcirculation was evaluated by fluorescent microscopy.

RESULTS:
From the 10 rats, 8 animal models were successfully established. The microcirculation of the liver could be clearly visualized by the fluorescent microscopy. The optimal concentration of fluorescent sodium was 0.1%. The direct and indirect phenomena caused by embolic material could be evaluated by fluorescent microscopy.

CONCLUSION:
Fluorescent microscopy with the corresponding Trans-hepatic arterial infusion animal model is a valuable method to evaluate the microcirculation of the liver and can be used for the evaluation of changes of liver microcirculation caused by embolization material.

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Author information

Author/s: Wang, Jian (J); Zou, Ying-hua (YH); Tong, Xiao-qiang (XQ); Lv, Yong-xing (YX); Jiang, Xue-xiang (XX);

Affiliation: Department of Interventional Radiology and Vascular Surgery, Peking University First Hospital, Beijing 100034, China.

Journal and publication information

Publication Type: English Abstract; Journal Article; Research Support, Non-U.S. Gov't

Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (Beijing Da Xue Xue Bao), published in China. (Language: chi)

Reference: 2006-Jun; vol 38 (issue 3) : pp 314-7

Dates: Created 2006/06/16; Completed 2008/06/11;

PMID: 16778980, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Embolization, Therapeutic - methods
Female
Fluorescent Dyes - administration & dosage
Hepatic Artery - physiology
Infusions, Intra-Arterial
Liver - blood supply

Male
Microcirculation
Microscopy, Fluorescence - methods
Models, Animal
Rats
Rats, Sprague-Dawley
Starch - administration & dosage

Associated Chemicals: Fluorescent Dyes (0) ; degradable starch microspheres (0) ; Starch (9005-25-8)

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