Research article summary (published 17 Jun 2006):

Critical roles of the immunoglobulin intronic enhancers in maintaining the sequential rearrangement of IgH and Igk loci.

Full Abstract

V(D)J recombination of immunoglobulin (Ig) heavy (IgH) and light chain genes occurs sequentially in the pro- and pre-B cells. To identify cis-elements that dictate this order of rearrangement, we replaced the endogenous matrix attachment region/Igk intronic enhancer (MiE(kappa)) with its heavy chain counterpart (Emu) in mice. This replacement, denoted EmuR, substantially increases the accessibility of both V(kappa) and J(kappa) loci to V(D)J recombinase in pro-B cells and induces Igk rearrangement in these cells. However, EmuR does not support Igk rearrangement in pre-B cells. Similar to that in MiE(kappa)(-/-) pre-B cells, the accessibility of V(kappa) segments to V(D)J recombinase is considerably reduced in EmuR pre-B cells when compared with wild-type pre-B cells. Therefore, Emu and MiE(kappa) play developmental stage-specific roles in maintaining the sequential rearrangement of IgH and Igk loci by promoting the accessibility of V, D, and J loci to the V(D)J recombinase.

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Author information

Author/s: Inlay, Matthew A (MA); Lin, Tongxiang (T); Gao, Heather H (HH); Xu, Yang (Y);

Affiliation: Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.

Grants: AI44838 (Agency:NIAID NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural

Journal: The Journal of experimental medicine (J Exp Med), published in United States. (Language: eng)

Reference: 2006-Jul; vol 203 (issue 7) : pp 1721-32

Dates: Created 2006/07/11; Completed 2006/08/22; Revised 2008/11/21;

PMID: 16785310, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals B-Lymphocytes - immunology; metabolism Enhancer Elements, Genetic - physiology Gene Rearrangement, B-Lymphocyte, Heavy Chain Gene Rearrangement, B-Lymphocyte, Light Chain Immunoglobulin Heavy Chains - genetics

Immunoglobulin Heavy Chains - genetics Immunoglobulin kappa-Chains - genetics Introns - physiology Mice Mice, Mutant Strains Stem Cells - immunology; metabolism

Associated Chemicals: Immunoglobulin Heavy Chains (0) ; Immunoglobulin kappa-Chains (0)

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