Polyelectrolyte coatings for microchip capillary electrophoresis.

Full Abstract

In chip-based electrophoretic analysis of biomolecules, chemical modification of the microchannel is widely employed to reduce or eliminate the analyte-wall interactions and alter electroosmotic flow (EOF) in the microchannel. A stable polyelectrolyte multilayer coating is one common way to regulate or eliminate EOF and prevent analyte adsorption for the rapid, efficient separation of biomolecules within microchannels. A wide variety of polyelectrolytes have been used as coatings. This chapter deals with how to coat microchips with polyelectrolytes and the expected results using polybrene and dextran sulfate as models. The technique presented here is generally applicable to any polyelectrolyte.

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Author information

Author/s: Liu, Yan (Y); Henry, Charles S (CS);

Affiliation: Department of Chemistry, Colorado State University, Fort Collins, CO, USA.

Journal and publication information

Publication Type: Comparative Study; Journal Article

Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol), published in United States. (Language: eng)

Reference: 2006-; vol 339 (issue ) : pp 57-64

Dates: Created 2006/06/22; Completed 2006/08/11; Revised 2006/11/15;

PMID: 16790867, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Dimethylpolysiloxanes
Electrochemistry
Electrolytes
Electrophoresis, Microchip - instrumentation; methods
Equipment Design

Associated Chemicals: Dimethylpolysiloxanes (0) ; Electrolytes (0) ; Nylons (0) ; Polymers (0) ; poly(dimethylsiloxane)-polyamide copolymer (0)

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