Measuring electroosmotic flow in microchips and capillaries.

Full Abstract

Electrophoretic migration and electroosmotic flow (EOF) combine to determine the migration rate of charged compounds in capillary electrophoresis (CE) and microchip capillary electrophoresis (MCE). Uncontrolled and unmeasured changes in EOF will lead to irreproducible peak migration times and poor peak quantitation. The two most common methods for measuring EOF for CE and MCE are detailed. Experimental results for application of the neutral marker method and the current monitoring method to EC are presented, and related calculations of EOF rates and electroosmotic mobility are described. The strengths and shortcomings of these two EOF measurement techniques are discussed. Additional approaches for studying and measuring EOF and for improving the reproducibility of migration times for CE and MCE are summarized.

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Author information

Author/s: Gilman, S Douglass (SD); Chapman, Peter J (PJ);

Affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, LA, USA.

Journal and publication information

Publication Type: Journal Article

Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol), published in United States. (Language: eng)

Reference: 2006-; vol 339 (issue ) : pp 187-202

Dates: Created 2006/06/22; Completed 2006/08/11;

PMID: 16790874, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Electrochemistry - methods
Electrophoresis, Capillary - methods

Electrophoresis, Microchip - methods
Osmosis

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