On-chip chiral separation based on bovine serum albumin-conjugated carbon nanotubes as stationary phase in a microchannel.

Full Abstract

A novel method of chiral separation based on protein-stationary phase immobilized in a poly(methyl methacrylate) microfluidic chip was developed. BSA conjugated with the shortened carboxylic single-walled carbon nanotubes (SWNTs) was employed as the chiral selector. Successful separation of tryptophan enantiomers was achieved in less than 70 s with a resolution factor of 1.35 utilizing a separation length of 32 mm. This is the first example of chiral separation based on SWNTs-BSA conjugates as stationary phase immobilized in microchip channel. The stability of the stationary phase in the channel was examined by microchip electrophoresis with laser-induced fluorescence detection. Factors that influenced the chiral separation resolution were examined. Under the optimized conditions, the proposed modified chip revealed adequate repeatability concerning run-to-run. These results show that the use of SWNTs-BSA conjugates within microfluidic channels hold great promise for a variety of analytical schemes.

Learn Faster Today Improve your study skills

Author information

Author/s: Weng, Xuexiang (X); Bi, Hongyan (H); Liu, Baohong (B); Kong, Jilie (J);

Affiliation: Department of Chemistry, Fudan University, Shanghai 200433, P. R. China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Electrophoresis (Electrophoresis), published in Germany. (Language: eng)

Reference: 2006-Aug; vol 27 (issue 15) : pp 3129-35

Dates: Created 2006/08/28; Completed 2006/12/12;

PMID: 16807934, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Carbon - chemistry
Electricity
Electrophoresis, Microchip - instrumentation; methods
Nanotechnology

Nanotechnology
Serum Albumin, Bovine - chemistry
Stereoisomerism
Tryptophan - chemistry

Associated Chemicals: Serum Albumin, Bovine (0) ; Tryptophan (73-22-3) ; Carbon (7440-44-0)

These are the highest related articles currently in the database:

See 100+ related articles.