Genetic filtering and optimal sampling of Schistosoma mansoni populations.

Full Abstract

Allelic variation in 6 microsatellite markers was compared between frozen Schistosoma mansoni eggs and laboratory-passaged worms originating from the same 5 fecal samples obtained from Brazilian residents. Based on allelic richness values, the number of alleles detected per locus did not differ between egg and worm DNA templates. However, our ability to score loci differed between these DNA templates, with worms providing more scored loci per individual than eggs. Differences also existed between the worms and eggs in the identity of the specific alleles that were detected. Additionally, we observed a reduction in homozygous genotypes among laboratory-passaged worms relative to the eggs. Allelic diversity curves were calculated by genotyping all worms from a representative host sample to determine the relationship between the number of alleles detected at a locus and the number of worms genotyped. Curves for the 5 residents' worm infrapopulations for each of the loci were very similar. The equation y=19.55 x ln(x) + 9.992 explained the association between sampling effort (x) and number of alleles detected (y) with an R(2) of 0.775. In conclusion, egg DNA templates and allelic diversity curves can benefit efforts to discern the sociological, ecological and evolutionary forces impacting the genetic diversity and disease epidemiology of human schistosomes.

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Author information

Author/s: Sorensen, R E (RE); Rodrigues, N B (NB); Oliveira, G (G); Romanha, A J (AJ); Minchella, D J (DJ);

Affiliation: Department of Biological Sciences, Minnesota State University Mankato, Mankato, MN 56001, USA. robert.sorensen(-atsign-)mnsu.edu

Grants: R01-AI42768 (Agency:NIAID NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: Parasitology (Parasitology), published in England. (Language: eng)

Reference: 2006-Oct; vol 133 (issue Pt 4) : pp 443-51

Dates: Created 2006/09/08; Completed 2006/11/09; Revised 2008/11/21;

PMID: 16817994, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Alleles
Animals
DNA, Helminth - genetics
Gene Frequency
Genetic Variation
Genotype

Genotype
Humans
Microsatellite Repeats - genetics
Schistosoma mansoni - genetics
Schistosomiasis mansoni - parasitology
Selection (Genetics)

Associated Chemicals: DNA, Helminth (0)

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