Adrenal expression of orexin receptor subtypes is differentially regulated in experimental streptozotocin induced type-1 diabetes.

Full Abstract

Orexins (hypocretins) are involved in the regulation of energy homeostasis and sleeping behavior. Orexins were also implicated in the regulation of neuroendocrine and autonomic functions. Recent data show the expression of orexin receptors within the hypothalamic-pituitary-adrenal (HPA) axis and suggest specific actions of orexins at the pituitary and adrenal glands. To further evaluate the role of orexin in the HPA axis, we investigated the mRNA expression of prepro-orexin (PPO) and orexin receptors within the HPA axis of streptozotocin-injected (STZ) rats showing type-1 like diabetes. PPO, as well as OX(1) and OX(2) receptor levels were analyzed by quantitative real-time PCR (qPCR). STZ rats were characterized by decreased body weight, plasma insulin, and leptin levels and by increased plasma glucose. Hypothalamic PPO mRNA levels were significantly reduced in STZ compared to non-diabetic control rats. No differences were found in the mRNA levels of hypothalamic or pituitary OX(1) and OX(2) receptors between control and STZ rats. In adrenals, OX(1) receptor mRNA levels were significantly elevated in STZ rats while OX(2) receptors were significantly reduced. Our results imply distinct functions of adrenal orexin receptor subtypes during type-1 like diabetes.

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Author information

Author/s: Jöhren, Olaf (O); Gremmels, Julia A F (JA); Qadri, Fatimunnisa (F); Dendorfer, Andreas (A); Dominiak, Peter (P);

Affiliation: Institute of Experimental and Clinical Pharmacology and Toxicology, University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany. Joehren(-atsign-)uni-luebeck.de

Journal and publication information

Publication Type: Journal Article

Journal: Peptides (Peptides), published in United States. (Language: eng)

Reference: 2006-Nov; vol 27 (issue 11) : pp 2764-9

Dates: Created 2006/10/23; Completed 2007/05/10;

PMID: 16822588, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adrenal Glands - metabolism
Animals
Blood Glucose - analysis
Body Weight
Diabetes Mellitus, Type 1 - chemically induced; metabolism
Hypothalamus - physiology
Insulin - blood
Intracellular Signaling Peptides and Proteins - blood; metabolism
Leptin - blood

Male
Neuropeptides - blood; metabolism
Pituitary Gland - physiology
RNA, Messenger - biosynthesis
Rats
Rats, Sprague-Dawley
Receptors, G-Protein-Coupled - genetics; metabolism
Receptors, Neuropeptide - genetics; metabolism
Streptozocin - pharmacology

Associated Chemicals: Blood Glucose (0) ; Intracellular Signaling Peptides and Proteins (0) ; Leptin (0) ; Neuropeptides (0) ; RNA, Messenger (0) ; Receptors, G-Protein-Coupled (0) ; Receptors, Neuropeptide (0) ; orexin receptors (0) ; orexins (0) ; Insulin (11061-68-0) ; Streptozocin (18883-66-4)

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