Early detection of Schistosoma mansoni infection by touchdown PCR in a mouse model.

Full Abstract

A detection assay for Schistosoma mansoni DNA in mouse serum samples based on touchdown PCR was developed and evaluated. The serum samples could be assayed directly without the need to extract DNA. No cross reactions between S. mansoni and related species inducing human schistosomiasis were observed. After the infection, mouse sera and feces were collected for 8 weeks. Anti-worm antigen IgG and anti-soluble egg antigen IgG were detected in the sera at 6 weeks post-infection by ELISA. The parasite's eggs were detected in the feces at 8 weeks. In contrast, S. mansoni DNA was detected in the sera at 2 weeks post-infection. These data suggest that touchdown PCR is a potential tool for the early diagnosis of S. mansoni infection.

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Author information

Author/s: Suzuki, Tomoyuki (T); Osada, Yoshio (Y); Kumagai, Takashi (T); Hamada, Atsuo (A); Okuzawa, Eiichi (E); Kanazawa, Tamotsu (T);

Affiliation: Department of Parasitology and Tropical Public Health, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Japan.

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.

Journal: Parasitology international (Parasitol Int), published in Netherlands. (Language: eng)

Reference: 2006-Sep; vol 55 (issue 3) : pp 213-8

Dates: Created 2006/07/24; Completed 2006/09/26; Revised 2006/11/15;

PMID: 16822708, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Antibodies, Helminth - blood; immunology
Antigens, Helminth - immunology
Early Diagnosis
Enzyme-Linked Immunosorbent Assay
Feces - parasitology
Female

Immunoglobulin G - blood; immunology
Mice
Parasite Egg Count
Polymerase Chain Reaction - methods
Schistosoma mansoni - genetics; immunology; isolation & purification
Schistosomiasis mansoni - diagnosis
Sensitivity and Specificity

Associated Chemicals: Antibodies, Helminth (0) ; Antigens, Helminth (0) ; Immunoglobulin G (0)

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