Research article summary (published 8 Jul 2006):

Cloning and functional characterization of 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (GbMECT) gene from Ginkgo biloba.

Full Abstract

2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (MECT), the third enzyme of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, catalyzes formation of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from MEP. GbMECT, presumably involved in ginkgolide biosynthesis, was cloned and characterized from Ginkgo biloba embryonic roots. The protein containing the N-terminal chloroplast transit peptide consisted of 327 amino acid residues. Complementation of GbMECT with Escherichia coli NMW33, ygbP (EcMECT) knock-out mutant, rescued the mutant, confirming the function of the protein. Transcription levels of GbMECT remained generally constant in embryonic roots and leaves for 1 month. Full 88 N-terminal residues were necessary to deliver the protein into the chloroplast as shown by protein-targeting analysis with GFP as a reporter protein in Arabidopsis thaliana protoplasts.

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Author information

Author/s: Kim, Sang-Min (SM); Kuzuyama, Tomohisa (T); Chang, Yung-Jin (YJ); Kwon, Hyung-Jin (HJ); Kim, Soo-Un (SU);

Affiliation: Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University, Sinlim-dong, Gwanak-gu, Seoul 151-921, Republic of Korea; Plant Metabolism Research Center, Kyung Hee University, Yongin 449-701, Republic of Korea.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Phytochemistry (Phytochemistry), published in United States. (Language: eng)

Reference: 2006-Jul; vol 67 (issue 14) : pp 1435-41

Dates: Created 2006/07/28; Completed 2006/11/13; Revised 2006/11/15;

PMID: 16828818, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Sequence Arabidopsis - enzymology; genetics Cell Line Cloning, Molecular DNA, Complementary - genetics Erythritol - analogs & derivatives; chemistry; metabolism Evolution, Molecular Gene Expression Regulation, Plant Ginkgo biloba - enzymology; genetics; growth & development

Ginkgolides - chemistry; metabolism Molecular Sequence Data Mutation - genetics Phosphotransferases - chemistry; genetics; metabolism Phylogeny Protoplasts - metabolism Sequence Alignment Sugar Phosphates - chemistry; metabolism Transcription, Genetic - genetics

Associated Chemicals: 2-C-methylerythritol 4-phosphate (0) ; DNA, Complementary (0) ; Ginkgolides (0) ; Sugar Phosphates (0) ; Erythritol (149-32-6) ; Phosphotransferases (EC 2.7.-)

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