Different levels of immunogenicity of two strains of Fowlpox virus as recombinant vaccine vectors eliciting T-cell responses in heterologous prime-boost vaccination strategies.

Full Abstract

The FP9 strain of F has been described as a more immunogenic recombinant vaccine vector than the Webster FPV-M (FPW) strain (R. J. Anderson et al., J. Immunol. 172:3094-3100, 2004). This study expands the comparison to include two separate recombinant antigens and multiple, rather than single, independent viral clones derived from the two strains. Dual-poxvirus heterologous prime-boost vaccination regimens using individual clones of recombinant FP9 or FPW in combination with recombinant modified V Ankara expressing the same antigen were evaluated for their ability to elicit T-cell responses against recombinant antigens from Plasmodium berghei (circumsporozoite protein) or human immunodeficiency virus type 1 (a Gag-Pol-Nef fusion protein). Gamma interferon enzyme-linked immunospot assay and fluorescence-activated cell sorting assays of the responses to specific epitopes confirmed the approximately twofold-greater cellular immunogenicity of FP9 compared to FPW, when given as the priming or boosting immunization. Equality of transgene expression in mouse cells infected with the two strains in vitro was verified by Western blotting. Directed partial sequence analysis and PCR analysis of FPW and comparison to available whole-genome sequences revealed that many loci that are mutated in the highly attenuated and culture-adapted FP9 strain are wild type in FPW, including the seven multikilobase deletions. These "passage-specific" alterations are hypothesized to be involved in determining the immunogenicity of fowlpox virus as a recombinant vaccine vector.

Learn Faster Today Improve your study skills

Author information

Author/s: Cottingham, Matthew G (MG); van Maurik, Andre (A); Zago, Manola (M); Newton, Angela T (AT); Anderson, Richard J (RJ); Howard, M Keith (MK); Schneider, Jörg (J); Skinner, Michael A (MA);

Affiliation: Department of Virology, Division of Investigative Science, Faculty of Medicine, Imperial College, St. Mary's Campus, Norfolk Place, London W2 1PG, United Kingdom.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Clinical and vaccine immunology : CVI (Clin Vaccine Immunol), published in United States. (Language: eng)

Reference: 2006-Jul; vol 13 (issue 7) : pp 747-57

Dates: Created 2006/07/10; Completed 2006/08/30; Revised 2008/11/21;

PMID: 16829611, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

AIDS Vaccines - genetics; immunology
Animals
Female
Fowlpox virus - classification; genetics; immunology
Fusion Proteins, gag-pol - genetics; immunology
Gene Products, nef - genetics; immunology; metabolism
Genetic Vectors - immunology
HIV-1 - genetics; immunology
Humans
Immunization, Secondary
Interferon-gamma - metabolism

Interferon-gamma - metabolism
Malaria Vaccines - genetics; immunology
Mice
Mice, Inbred BALB C
Plasmodium berghei - genetics; immunology
Polyproteins - genetics; immunology
Protozoan Proteins - genetics; immunology
T-Lymphocytes - immunology
Vaccination
Vaccines, DNA - immunology
nef Gene Products, Human Immunodeficiency Virus

Associated Chemicals: AIDS Vaccines (0) ; Fusion Proteins, gag-pol (0) ; Gene Products, nef (0) ; Malaria Vaccines (0) ; Polyproteins (0) ; Protozoan Proteins (0) ; Vaccines, DNA (0) ; nef Gene Products, Human Immunodeficiency Virus (0) ; Interferon-gamma (82115-62-6)

These are the highest related articles currently in the database:

See 100+ related articles.