Nonaqueous capillary electrophoresis coupled with laser-induced native fluorescence detection for the analysis of berberine, palmatine, and jatrorrhizine in Chinese herbal medicines.

Full Abstract

LIF detection is one of the most sensitive detection methods for CE. However, its application is limited because the analyte is usually required to be derivatized with a fluorescent label. As a result, LIF is seldom used to analyze active ingredients in plants. In this work, we introduce a rapid, simple, and sensitive method of nonaqueous CE (NACE) coupled with laser-induced native fluorescence detection for the simultaneous analysis of berberine, palmatine, and jatrorrhizine. This method skillfully utilizes the native fluorescence of these alkaloids and requires no troublesome fluorescent derivatization. As these alkaloids can fluoresce to some degree, they were simply detected by a commercially available 488 nm Ar+ laser. The native fluorescence of the analytes was greatly enhanced by nonaqueous media. Compared with the reported UV detection method, much lower LOD was achieved (6.0 ng/mL for berberine, 7.5 ng/mL for palmatine, and 380 ng/mL for jatrorrhizine). This method was successfully applied to analyze berberine, palmatine, and jatrorrhizine in two Chinese herbal medicines, Rhizoma coptidis and Caulis mahoniae.

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Author information

Author/s: Liu, Qian (Q); Liu, Yingju (Y); Li, Yangqing (Y); Yao, Shouzhuo (S);

Affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P. R. China.

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of separation science (J Sep Sci), published in Germany. (Language: eng)

Reference: 2006-Jun; vol 29 (issue 9) : pp 1268-74

Dates: Created 2006/07/12; Completed 2007/12/17;

PMID: 16833085, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acetates - chemistry
Acetic Acid - chemistry
Berberine - analogs & derivatives; analysis
Berberine Alkaloids - analysis
Drugs, Chinese Herbal - chemistry
Electrophoresis, Capillary - instrumentation; methods
Lasers

Medicine, Chinese Traditional
Molecular Structure
Plant Extracts - chemistry
Plants, Medicinal - chemistry
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence - methods

Associated Chemicals: Acetates (0) ; Berberine Alkaloids (0) ; Drugs, Chinese Herbal (0) ; Plant Extracts (0) ; Berberine (2086-83-1) ; palmatine (3486-67-7) ; jatrorrhizine (3621-38-3) ; ammonium acetate (631-61-8) ; Acetic Acid (64-19-7)

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