Research article summary (published 10 Jun 2006):

Comparison of [18F]FDOPA, [18F]FMT and [18F]FECNT for imaging dopaminergic neurotransmission in mice.

Full Abstract

INTRODUCTION:
The clinically established positron emission tomography (PET) tracers 6-[(18)F]-fluoro-l-DOPA ([(18)F]FDOPA), 6-[(18)F]-fluoro-l-m-tyrosine ([(18)F]FMT) and 2beta-carbomethoxy-3beta-(4-chlorophenyl)-8-(2-[(18)F]-fluoroethyl)-nortropane ([(18)F]FECNT) serve as markers of presynaptic integrity of dopaminergic nerve terminals in humans. This study describes our efforts to adopt the methodology of human Parkinson's disease (PD) PET studies to mice.

METHODS:
The PET imaging characteristics of [(18)F]FDOPA, [(18)F]FMT and [(18)F]FECNT were analyzed in healthy C57BL/6 mice using the dedicated small-animal PET tomograph quad-HIDAC. Furthermore, [(18)F]FECNT was tested in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of PD.

RESULTS:
[(18)F]FDOPA and [(18)F]FMT failed to clearly visualize the mouse striatum, whereas PET experiments using [(18)F]FECNT proved that the employed methodology is capable of delineating the striatum in mice with exquisite resolution. Moreover, [(18)F]FECNT PET imaging of healthy and MPTP-lesioned mice demonstrated that the detection and quantification of striatal degeneration in lesioned mice can be accomplished.

CONCLUSIONS:
This study shows the feasibility of using [(18)F]FECNT PET to analyze noninvasively the striatal degeneration in the MPTP mouse model of PD. This methodology can be therefore considered as a viable complement to established in vivo microdialysis and postmortem techniques.

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Author information

Author/s: Honer, Michael (M); Hengerer, Bastian (B); Blagoev, Milen (M); Hintermann, Samuel (S); Waldmeier, Peter (P); Schubiger, Pius A (PA); Ametamey, Simon M (SM);

Affiliation: Center for Radiopharmaceutical Science of ETH, PSI and USZ, CH-8093 Zurich, Switzerland. michael.honer(-atsign-)pharma.ethz.ch

Journal and publication information

Publication Type: Comparative Study; Journal Article

Journal: Nuclear medicine and biology (Nucl Med Biol), published in England. (Language: eng)

Reference: 2006-Jul; vol 33 (issue 5) : pp 607-14

Dates: Created 2006/07/17; Completed 2006/10/06; Revised 2006/11/15;

PMID: 16843835, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Corpus Striatum - metabolism; radionuclide imaging Dihydroxyphenylalanine - analogs & derivatives; diagnostic use; pharmacokinetics Dopamine - metabolism Male Mice Mice, Inbred C57BL Neurotransmitter Agents - metabolism Nortropanes - diagnostic use; pharmacokinetics

Nortropanes - diagnostic use; pharmacokinetics Parkinson Disease - metabolism; radionuclide imaging Parkinsonian Disorders - metabolism; radionuclide imaging Radiopharmaceuticals - diagnostic use; pharmacokinetics Reproducibility of Results Sensitivity and Specificity Substantia Nigra - metabolism; radionuclide imaging Synaptic Transmission Tyrosine - analogs & derivatives; diagnostic use; pharmacokinetics

Associated Chemicals: 2-carbomethoxy-3-(4-chlorophenyl)-8-(2-fluoroethyl)nortropane (0) ; Neurotransmitter Agents (0) ; Nortropanes (0) ; O-(18F)fluoromethyl-L-tyrosine (0) ; Radiopharmaceuticals (0) ; Dopamine (51-61-6) ; Tyrosine (55520-40-6) ; Dihydroxyphenylalanine (63-84-3) ; fluorodopa F 18 (75290-51-6)

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