Analysis of hop acids and their oxidized derivatives and iso-alpha-acids in beer by capillary electrophoresis-electrospray ionization mass spectrometry.

Full Abstract

This study investigates the applicability of on-line coupling of capillary electrophoresis with electrospray ionization tandem mass spectrometry (CZE-ESI-MS) for the separation and characterization of alpha- and beta-acids and oxidized hop acids from crude extracts of different hop varieties. CZE-ESI-MS with negative-ion electrospray ionization proved to be a suitable technique for the determination of these types of natural compounds and their oxidized derivatives. The CZE parameters (pH, concentration, and buffer type) and ESI-MS parameters (nature and flow rate of the sheath liquid, nebulizer pressure, drying gas flow rate, temperature, and compound stability) were optimized. The optimized method provides the potential for a fast qualitative determination of hop acids and their oxidation compounds. The method was also applied to the determination of iso-alpha-acids in beer.

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Author information

Author/s: García-Villalba, Rocío (R); Cortacero-Ramírez, Sonia (S); Segura-Carretero, Antonio (A); Martín-Lagos Contreras, José Antonio (JA); Fernández-Gutiérrez, Alberto (A);

Affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, c/ Fuentenueva s/n, 18071 Granada, Spain.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of agricultural and food chemistry (J Agric Food Chem), published in United States. (Language: eng)

Reference: 2006-Jul; vol 54 (issue 15) : pp 5400-9

Dates: Created 2006/07/19; Completed 2006/08/29; Revised 2006/11/15;

PMID: 16848524, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Beer - analysis
Cyclohexenes
Electrophoresis, Capillary
Humulus - chemistry

Humulus - chemistry
Hydrogen-Ion Concentration
Spectrometry, Mass, Electrospray Ionization
Terpenes - analysis

Associated Chemicals: Cyclohexenes (0) ; Terpenes (0) ; humulon (469-02-3)

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