Highly efficient dynamic modification of plastic microfluidic devices using proteins in microchip capillary electrophoresis.

Full Abstract

New dynamic coating agents were investigated for the manipulation of electroosmotic flow (EOF) in poly(methylmethacrylate) (PMMA) microchips. Blocking proteins designed for enzyme-linked immunosorbent assay (ELISA) applications (e.g. Block Ace and UltraBlock), and egg-white lysozyme were proposed in this study. The EOF could be enhanced, suppressed or its direction could be reversed, depending on the buffer pH and the charge on the proteins. The coating procedure is simple, requiring only filling of the microchannels with a coating solution, followed by a rinse with a running buffer solution prior to analysis. One major advantage of this method is that it is not necessary to add the coating agent to the running buffer solution. Block Ace and UltraBlock coatings were stable for at least five runs in a given microchannel without the need to condition the coating between runs other than replenishing the buffer solution after each run, i.e. the RSD values of EOF (n=5) were less than 4.3%, and there was no significant change in the EOF after 5 runs. The reproducibility of the coating procedures was found from the channel-to-channel RSD values of the EOF, and were less than 5.0% when using HEPES-Na buffer (pH 7.4) as the running buffer. Several examples of electrophoretic separations of amino acids and biogenic amines derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) are demonstrated in this paper. The dynamic coating method has the potential for a broad range of applications in microchip capillary electrophoresis (microchip CE) separations.

Learn Faster Today Improve your study skills

Author information

Author/s: Naruishi, Nahoko (N); Tanaka, Yoshihide (Y); Higashi, Tetsuji (T); Wakida, Shin-ichi (S);

Affiliation: Human Stress Signal Research Center, HSS, National Institute of Advanced Industrial Science and Technology, AIST, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.

Journal and publication information

Publication Type: Journal Article

Journal: Journal of chromatography. A (J Chromatogr A), published in Netherlands. (Language: eng)

Reference: 2006-Oct; vol 1130 (issue 2) : pp 169-74

Dates: Created 2006/10/02; Completed 2006/11/22;

PMID: 16860810, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amphetamine - analysis; chemistry
Electrophoresis, Microchip - instrumentation; methods
Gas Chromatography-Mass Spectrometry - methods
Hair - chemistry
Humans
Indicators and Reagents - chemistry

Indicators and Reagents - chemistry
Methamphetamine - analysis; chemistry
Microfluidics - instrumentation; methods
Molecular Structure
Proteins - analysis; chemistry
Reproducibility of Results

Associated Chemicals: Indicators and Reagents (0) ; Proteins (0) ; Amphetamine (300-62-9) ; Methamphetamine (537-46-2)

These are the highest related articles currently in the database:

See 100+ related articles.