Expression of secretory leukocyte protease inhibitor in middle ear cholesteatoma.

Full Abstract

Secretory leukocyte protease inhibitor (SLPI) is a cationic protein and a member of the innate immunity-associated protein family. The main function of SLPI is to protect local tissue against the detrimental consequences of inflammation. We undertook this study to investigate the expression of SLPI in human middle ear cholesteatoma tissue as compared with normal external auditory canal skin. Cholesteatoma tissues and external auditory canal skin samples were obtained from eight patients during middle ear surgery. The expression levels of SLPI mRNA and protein were evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. Immunohistochemical staining was performed in both tissue groups using anti-SLPI antibody. Real-time RT-PCR showed that SLPI mRNA levels in cholesteatoma tissues were increased 7.8-fold on average as compared with normal auditory canal skin. Western blotting analysis showed that SLPI protein expression in cholesteatoma epithelium is up-regulated versus external auditory canal skin epithelium. Immunohistochemical staining for SLPI showed that SLPI is expressed mainly in the stratum granulosum and in subepithelial inflammatory cells. These findings imply that SLPI contributes to host protection against inflammatory cell and destructive enzymes in the chronic inflammatory state of cholesteatoma by affecting the innate immune system.

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Author information

Author/s: Lee, Jung-Kyu (JK); Chae, Sung-Won (SW); Cho, Jae-Gu (JG); Lee, Heung-Man (HM); Hwang, Soon-Jae (SJ); Jung, Hak-Hyun (HH);

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Guro Hospital, Korea University College of Medicine, 80 Guro-dong, Guro-gu, Seoul, South Korea. schae(-atsign-)kumc.or.kr

Journal and publication information

Publication Type: Journal Article

Journal: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (Eur Arch Otorhinolaryngol), published in Germany. (Language: eng)

Reference: 2006-Dec; vol 263 (issue 12) : pp 1077-81

Dates: Created 2006/11/06; Completed 2007/10/25;

PMID: 16868773, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult
Aged
Blotting, Western
Cholesteatoma, Middle Ear - genetics; metabolism; pathology
Humans

Immunohistochemistry
Middle Aged
RNA, Messenger - genetics
Reverse Transcriptase Polymerase Chain Reaction
Secretory Leukocyte Peptidase Inhibitor - genetics; metabolism

Associated Chemicals: RNA, Messenger (0) ; Secretory Leukocyte Peptidase Inhibitor (0)

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