Comparision of two-flow cytometry methods for basophil degranulation in patients sensitized to grass pollen.

Full Abstract

BACKGROUND: Two-flow cytometry methods for quantification of degranulated basophil after allergen-specific activation were discussed. The methods are discerned by used membrane receptors--FcepsilonRI or IL-3Ralpha Our goal was to evaluate the diagnostic potential of the methods and to correlate them to allergen-specific IgE detection and skin prick test (SPT). METHODS: Patient's and control's groups were studied with Bulgarian grass pollen allergen B1 simultaneously by flow cytometry kits: Basotest and BD FastImmune test. Allergy diagnosis was based on clinical history and SPT. The determination of specific IgE was performed by ELISA--RIDASCREEN. RESULTS: There were no significant differences between the patient's results from Basotest and FastImmune (P>0.05). A significant correlation between values, analyzed by Basotest and by FastImmune was found (r=0.88). The sensitivity and specificity of the Basotest, FastImmune, specific IgE and SPT were 85%, 72%, 92% and 92% sensitivity and 100%, 92%, 100% and 85% specificity respectively. The efficiency was between 82% and 97%. There were a significant correlation between the specific IgE and flow cytometry tests: tau=0.92 (Basotest) and tau=0.71 (FastImmune) and a moderate significant correlation between the SPT and the in vitro tests: tau=0.26 (Basotest) and tau=0.31 (FastImmune). CONCLUSION: The successful use of the Bulgarian grass pollen allergen B1 and both flow cytometry tests was presented. These methods could be as specific tools for IgE-mediated diagnosis especially FastImmune in the case of low IgE receptor expression.

Author information

Author/s: Michova, A (A); Abugalia, M (M); Ivanova, Ts (Ts); Nikolov, G (G); Taskov, H (H); Petrunov, B (B);

Affiliation: Central Laboratory of Immunology, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria.

Journal and publication information

Publication Type: Comparative Study; Journal Article

Journal: Allergy (Allergy), published in Denmark. (Language: eng)

Reference: 2006-Sep; vol 61 (issue 9) : pp 1078-83

Dates: Created 2006/08/21; Completed 2007/01/10;

PMID: 16918510, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

Comments and Corrections

CommentIn: Allergy. 2006 Sep;61(9):1025-7. (PMID: 16918503)

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