Quantitative determination of major active components in Ginkgo biloba dietary supplements by liquid chromatography/mass spectrometry.

Full Abstract

A reversed-phase high-performance liquid chromatography/electrospray ionisation mass spectrometry (RP-HPLC/ESI-MS) method was developed and validated for the simultaneous determination of ten major active components in Ginkgo biloba extract (bilobalide, ginkgolides A, B, C, quercetin, kaempferol, isorhamnetin, rutin hydrate, quercetin-3-beta-D-glucoside and quercitrin hydrate) which have not been previously reported to be quantified in a single analysis. The ten components exhibit baseline separation in 50 min by C18 chromatography using a water/1:1 (v/v) methanol/acetonitrile gradient. Quantitation was performed using negative ESI-MS in selected ion monitoring (SIM) mode. Good reproducibility and recovery were obtained by this method. The sensitivity of both UV and different mass spectrometry modes (full scan, selected ion monitoring (SIM), and selected reaction monitoring (SRM)) were compared and both quantitation with and without internal standard were evaluated. The analysis of Ginkgo biloba commercial products showed remarkable variations in the rutin and quercetin content as well as the terpene lactone contents although all the products satisfy the conventional quality control method.Copyright 2006 John Wiley & Sons, Ltd.

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Author information

Author/s: Ding, Shujing (S); Dudley, Ed (E); Plummer, Sue (S); Tang, Jiandong (J); Newton, Russell P (RP); Brenton, A Gareth (AG);

Affiliation: Mass Spectrometry Research Unit, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.

Journal and publication information

Publication Type: Journal Article; Validation Studies

Journal: Rapid communications in mass spectrometry : RCM (Rapid Commun Mass Spectrom), published in England. (Language: eng)

Reference: 2006-; vol 20 (issue 18) : pp 2753-60

Dates: Created 2006/09/04; Completed 2007/08/30;

PMID: 16921563, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Chromatography, High Pressure Liquid
Cyclopentanes - analysis
Dietary Supplements - analysis
Flavonols - analysis
Furans - analysis
Ginkgo biloba

Ginkgolides - analysis
Kaempferols - analysis
Plant Extracts - analysis
Quercetin - analogs & derivatives; analysis
Rutin - analysis
Spectrometry, Mass, Electrospray Ionization - methods

Associated Chemicals: Cyclopentanes (0) ; Flavonols (0) ; Furans (0) ; Ginkgolides (0) ; Kaempferols (0) ; Plant Extracts (0) ; quercetin-3-(6''-galloyl)glucoside (0) ; Quercetin (117-39-5) ; Rutin (153-18-4) ; bilobalide (33570-04-6) ; 3-methylquercetin (480-19-3) ; kaempferol (520-18-3)

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