Separation and characterization of rabbit liver apothioneins by capillary electrophoresis coupled to electrospray ionization time-of-flight mass spectrometry.

Full Abstract

The present study establishes a method for the separation and characterization of rabbit liver metallothionein (MT) subisoforms by capillary electrophoresis coupled to electrospray ionization time-of-flight mass spectrometry (CE-ESI-TOF-MS) via a sheath-flow interface. Directly coupled-CE-MS enables the extraction of specific molecular weight information and thereby facilitates the identification of peaks when no reference materials are available, as in the case of MT subisoforms. The analysis described here revealed the presence of the apothioneins MT-1a, MT-2d, and MT-2e, belonging to MT-I sample, and MT-2a, MT-2b, and MT-2c, belonging to MT-II. Several non-N-acetylated forms were also detected as traces appearing with their respective acetylated forms in both samples. Similar results were found when MALDI-TOF experiments were performed, identifying all the sequenced rabbit liver MTs as apo-MT-forms, as in the CE-ESI-MS coupling.

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Author information

Author/s: Andón, Balbina (B); Barbosa, José (J); Sanz-Nebot, Victoria (V);

Affiliation: Departament Química Analítica, Facultat de Química, Universitat de Barcelona, Barcelona, Spain.

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: Electrophoresis (Electrophoresis), published in Germany. (Language: eng)

Reference: 2006-Sep; vol 27 (issue 18) : pp 3661-70

Dates: Created 2006/09/20; Completed 2006/12/14;

PMID: 16927421, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Sequence
Animals
Apoproteins - analysis; isolation & purification
Electrophoresis, Capillary - methods
Liver - chemistry

Liver - chemistry
Metallothionein - analysis; isolation & purification
Molecular Sequence Data
Rabbits
Spectrometry, Mass, Electrospray Ionization - methods

Associated Chemicals: Apoproteins (0) ; apothionein (0) ; Metallothionein (9038-94-2)

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