Expression profiles of Na+,K+-ATPase during acute and chronic hypo-osmotic stress in the blue crab Callinectes sapidus.

Full Abstract

During acclimation to dilute seawater, the specific activity of Na+,K+-ATPase increases substantially in the posterior gills of the blue crab Callinectes sapidus. To determine whether this increase occurs through regulation of pre-existing enzyme or synthesis of new enzyme, mRNA and protein levels were measured over short (<24 h) and long (18 days) time courses. Na+,K+-ATPase expression, both mRNA and protein, did not change during the initial 24-h exposure to dilute seawater (10 ppt salinity). Thus, osmoregulation in C. sapidus during acute exposure to low salinity likely involves either modulation of existing enzyme or mechanisms other than an increase in the amount of Na+,K+-ATPase enzyme. However, crabs exposed to dilute seawater over 18 days showed a 300% increase in Na+,K+-ATPase specific activity as well as a 200% increase in Na+,K+-ATPase protein levels. Thus, it appears that the increase in Na+,K+-ATPase activity during chronic exposure results from the synthesis of new enzyme. The relative amounts of mRNA for the alpha-subunit increased substantially (by 150%) during the acclimation process, but once the crabs had fully acclimated to low salinity, the mRNA levels had decreased and were not different from levels in crabs fully acclimated to high salinity. Thus, there is transient induction of the Na+,K+-ATPase mRNA levels during acclimation to dilute seawater.

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Author information

Author/s: Lovett, Donald L (DL); Verzi, Michael P (MP); Burgents, Joseph E (JE); Tanner, Christopher A (CA); Glomski, Krzysztof (K); Lee, Joan J (JJ); Towle, David W (DW);

Affiliation: Department of Biology, The College of New Jersey, Ewing, New Jersey 08628, USA. lovett(-atsign-)tcnj.edu

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.

Journal: The Biological bulletin (Biol Bull), published in United States. (Language: eng)

Reference: 2006-Aug; vol 211 (issue 1) : pp 58-65

Dates: Created 2006/09/01; Completed 2007/01/19; Revised 2008/11/21;

PMID: 16946242, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Brachyura - enzymology
Gene Expression Profiling
Gene Expression Regulation, Enzymologic

Osmolar Concentration
RNA, Messenger
Sodium-Potassium-Exchanging ATPase - metabolism
Stress, Physiological - enzymology

Associated Chemicals: RNA, Messenger (0) ; Sodium-Potassium-Exchanging ATPase (EC 3.6.3.9)

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