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| Research article summary (published 29 Nov 2006): |
5-Hydroxytryptamine-mediated increase in glutamate uptake by the leech giant glial cell.
Full Abstract
The clearance of synaptically released glutamate is one of the pivotal functions of glial cells. We have studied the role of 5-hydroxytryptamine (5-HT, 30 microM), a neurotransmitter and neurohormone in the leech central nervous system with a versatile action spectrum, on the efficacy of glial glutamate uptake. The activity of the glutamate uptake carrier in the giant glial cell in isolated ganglia of Hirudo medicinalis was monitored by measuring the membrane current and the change in the intracellular Na(+) concentration (Na(+) (i)) as induced by the glutamate carrier substrate D-aspartate (D-asp, 1 mM). 5-HT increased the D-asp-induced current (EC(50) at 5 microM) and rise in Na(+) (i), an effect which was mimicked by the membrane-permeable cyclic nucleotide analogue dibutyryl-cyclic AMP (db-cAMP). The adenylyl cyclase inhibitor SQ 22,536 and the protein kinase A antagonist Rp-cAMP inhibited the effect of 5-HT. Blocking the G protein in the giant glial cell by injecting GDP-beta-S suppressed the effect of 5-HT, but not the effect of db-cAMP, on the D-asp-induced current. Our results suggest that 5-HT enhances the glial uptake of glutamate via cAMP- and PKA-mediated pathway. (c) 2006 Wiley-Liss, Inc.
Author information
Author/s: Hirth, Ingolf C (IC); Deitmer, Joachim W (JW);
Affiliation: Abteilung für Allgemeine Zoologie, FB Biologie, Universität Kaiserslautern, Postfach 3049, D-67653 Kaiserslautern, Germany.
Journal and publication information
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal: Glia (Glia), published in United States. (Language: eng)
Reference: 2006-Dec; vol 54 (issue 8) : pp 786-94
Dates: Created 2006/10/03; Completed 2007/01/05; Revised 2007/11/15;
PMID: 16958089, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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