Research article summary (published 3 Oct 2006):

DeepSAGE--digital transcriptomics with high sensitivity, simple experimental protocol and multiplexing of samples.

Full Abstract

Digital transcriptomics with pyrophosphatase based ultra-high throughput DNA sequencing of di-tags provides high sensitivity and cost-effective gene expression profiling. Sample preparation and handling are greatly simplified compared to Serial Analysis of Gene Expression (SAGE). We compare DeepSAGE and LongSAGE data and demonstrate greater power of detection and multiplexing of samples derived from potato. The transcript analysis revealed a great abundance of up-regulated potato transcripts associated with stress in dormant potatoes compared to harvest. Importantly, many transcripts were detected that cannot be matched to known genes, but is likely to be part of the abiotic stress-response in potato.

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Author information

Author/s: Nielsen, Kåre L (KL); Høgh, Annabeth Laursen (AL); Emmersen, Jeppe (J);

Affiliation: Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University DK-9000 Aalborg, Denmark.

Journal and publication information

Publication Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: Nucleic acids research (Nucleic Acids Res), published in England. (Language: eng)

Reference: 2006-; vol 34 (issue 19) : pp e133

Dates: Created 2006/11/07; Completed 2006/12/20; Revised 2008/11/20;

PMID: 17028099, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Diphosphates - chemistry Gene Expression Profiling - methods Gene Expression Regulation, Plant RNA, Messenger - analysis

RNA, Messenger - analysis Sequence Analysis, DNA Sequence Tagged Sites Solanum tuberosum - genetics; metabolism

Associated Chemicals: Diphosphates (0) ; RNA, Messenger (0)

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