Direct high-resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry.

Full Abstract

We report the first coupling of a desorption electrospray ionization (DESI) ion source to Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS) for high-resolution protein analysis. The DESI FT-ICR-MS source design is described in detail along with preliminary data obtained on peptides and proteins ranging from 1 to 5.7 kDa.Copyright 2006 John Wiley & Sons, Ltd.

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Author information

Author/s: Bereman, Michael S (MS); Nyadong, Leonard (L); Fernandez, Facundo M (FM); Muddiman, David C (DC);

Affiliation: W M Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.

Grants: R33 CA105295 (Agency:NCI NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: Rapid communications in mass spectrometry : RCM (Rapid Commun Mass Spectrom), published in England. (Language: eng)

Reference: 2006-; vol 20 (issue 22) : pp 3409-11

Dates: Created 2006/11/02; Completed 2007/01/19; Revised 2007/12/03;

PMID: 17051610, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Bradykinin - analysis
Formic Acids - analysis
Glucagon - analysis
Insulin - analysis
Melitten - analysis

Peptides - analysis
Proteins - analysis
Spectrometry, Mass, Electrospray Ionization - methods
Spectroscopy, Fourier Transform Infrared
Vasodilator Agents - analysis

Associated Chemicals: Formic Acids (0) ; Peptides (0) ; Proteins (0) ; Vasodilator Agents (0) ; Insulin (11061-68-0) ; Melitten (20449-79-0) ; Bradykinin (58-82-2) ; formic acid (64-18-6) ; Glucagon (9007-92-5)

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