Mediators of rat ischemic hepatic preconditioning after cold preservation identified by microarray analysis.

Full Abstract

Hepatic ischemia-reperfusion injury associated with liver transplantation is an as yet unresolved problem in clinical practice. Preconditioning protects the liver against the deleterious effects of ischemia, although the mechanism underlying this preconditioning is still unclear. To profile gene expression patterns involved in hepatic ischemic preconditioning, we analyzed the changes in gene expression in rat livers by DNA microarray analysis. Approximately 116 genes were found to have altered gene expression after 8 hours of cold ischemia. Moreover, the expression of 218 genes was modified by classic preconditioning followed by the same ischemia process. Given the importance of the effects of ischemic preconditioning (IP) in minimizing the liver damage induced by sustained ischemia before reperfusion, this study analyzed the putative genes involved in the beneficial role of IP in liver grafts undergoing cold ischemia before its implantation in the recipient (IP+I). Great differences were found in the gene expression pattern of ischemic preconditioning + long cold ischemia (IP+I) group when compared with the long cold ischemia alone condition (I), which could explain the protective regulatory mechanisms that take place after preconditioning. Twenty-six genes that were downregulated in cold ischemia were found upregulated after preconditioning preceding a long cold ischemia period. These would be genes activated or maintained by preconditioning. Heat shock protein genes and 3-hydroxy-3-methylglutaryl-coenzyme A reductase are among the most markedly induced transcripts.(c) 2006 AASLD

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Author information

Author/s: Navarro-Sabaté, Aurea (A); Peralta, Carmen (C); Calvo, Maria Nieves (MN); Manzano, Anna (A); Massip-Salcedo, Marta (M); Roselló-Catafau, Joan (J); Bartrons, Ramon (R);

Affiliation: Unitat de Bioquímica, Departament de Ciències Fisiològiques II, Campus de Bellvitge, IDIBELL-Universitat de Barcelona, Feixa Llarga s/n E-08907 L'Hospitalet, Spain.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society (Liver Transpl), published in United States. (Language: eng)

Reference: 2006-Nov; vol 12 (issue 11) : pp 1615-25

Dates: Created 2006/11/01; Completed 2007/01/30;

PMID: 17058268, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Cold Ischemia
Computer Systems
Down-Regulation
Gene Expression
Gene Expression Profiling
Gene Expression Regulation
Heat-Shock Proteins - genetics
Hydroxymethylglutaryl CoA Reductases - genetics
Ischemic Preconditioning

Ischemic Preconditioning
Liver - blood supply; metabolism
Male
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
RNA, Messenger - metabolism
Rats
Rats, Sprague-Dawley
Time Factors
Up-Regulation

Associated Chemicals: Heat-Shock Proteins (0) ; RNA, Messenger (0) ; Hydroxymethylglutaryl CoA Reductases (EC 1.1.1.-)

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