Anabolic effects of a non-myotoxic dose of the beta2-adrenergic receptor agonist clenbuterol on rat plantaris muscle.

Full Abstract

Previous investigations of the effects of clenbuterol have used suprapharmacological doses that induce myocyte death, alter muscle phenotype, and do not approximate the proposed therapeutic dose for humans. Recently, we reported that smaller doses of clenbuterol induce muscle growth without causing myocyte death. In the present study we used histochemical and proteomic techniques to investigate the molecular effects of this dose. Male Wistar rats (n = 6, per group) were infused with saline or 10 microg/kg/day clenbuterol via subcutaneously implanted osmotic pumps. After 14 days the animals' plantaris muscles were isolated for histochemical and proteomic analyses. Clenbuterol induced significant muscle growth with concomitant protein accretion and preferential hypertrophy of fast oxidative glycolytic fibers. Clenbuterol reduced the optical density of mitochondrial staining in fast fibers by 20% and the glycogen content of the muscle by 30%. Differential analysis of two-dimensional gels showed that heat shock protein 72 and beta-enolase increased, whereas aldolase A, phosphogylcerate mutase, and adenylate kinase decreased. Only heat shock protein 72 has previously been investigated in clenbuterol-treated muscles. The clenbuterol-induced increase in muscle growth was concomitant with qualitative changes in the muscle's proteome that need to be considered when proposing therapeutic uses for this agent.

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Author information

Author/s: Burniston, Jatin G (JG); McLean, Lynn (L); Beynon, Robert J (RJ); Goldspink, David F (DF);

Affiliation: Muscle Physiology and Proteomics Laboratory, Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Webster Street, Liverpool L3 2ET, UK. j.burniston(-atsign-)ljmu.ac.uk

Grants: FS/04/028/16327 (Agency:British Heart Foundation)

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Muscle & nerve (Muscle Nerve), published in United States. (Language: eng)

Reference: 2007-Feb; vol 35 (issue 2) : pp 217-23

Dates: Created 2007/01/24; Completed 2007/03/27; Revised 2008/11/21;

PMID: 17058275, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adrenergic beta-Agonists - pharmacology
Animals
Clenbuterol - pharmacology
Dose-Response Relationship, Drug
Electrophoresis, Gel, Two-Dimensional - methods
Male

Muscle Fibers, Skeletal - drug effects
Muscle Proteins - metabolism
Muscle, Skeletal - drug effects; metabolism
Proteomics - methods
Rats
Rats, Wistar

Associated Chemicals: Adrenergic beta-Agonists (0) ; Muscle Proteins (0) ; Clenbuterol (37148-27-9)

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