[Expression of p27 in rat kidney with unilateral ureteral obstruction and the therapeutic effect of enalapril]

Full Abstract

OBJECtive: To explore the effect of p27 in the renal tubule on the process of renal interstitial fibrosis caused by unilateral ureteral obstruction (UUO) in rats, and to examine the expression changes of p27 after enalapril intervention and to interpret the anti-fibrotic mechanism. METHODS: Ninety rats were randomly divided into the sham-operated group (SOR), UUO group,and UUO+enalapril treatment group [enalapril: 10 mg/(kg.d)]. The rats of each group were respectively sacrificed on 7, 14, 21 days post-operatively. The renal pathological changes were dynamically observed by HE. The expression and dynamic changes of p27 were detected by immunohistochemistry. The level of p27 mRNA were detected by RT-PCR. RESULTS: The expression of p27 in renal tubular epithelial cells and p27 mRNA were strongly positive in the SOR group. With degree of interstitial fibrosis aggravating, the expression of p27 mRNA was gradually reducing. Enalapril could improve the expression of p27 on the 14th and 21st days after the UUO. CONCLUSION: (1) This study supports a causative role of p27 in the formation of fibrosis of renal mesenchyme in rats with UUO. (2) The anti-fibrotic mechanism of enalapril is partly the improvement of p27 expression.

Author information

Author/s: Sun, Jian (J); Tao, Li-jian (LJ); Jin, Ou (O); Ning, Wang-bin (WB); Damu, Tang (T);

Affiliation: Department of Nephrology, Xiangya Hospital, Central South University, Changsha 410008, China.

Journal and publication information

Publication Type: English Abstract; Journal Article

Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban), published in China. (Language: chi)

Reference: 2006-Oct; vol 31 (issue 5) : pp 671-5

Dates: Created 2006/10/25; Completed 2007/03/29;

PMID: 17062928, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Angiotensin-Converting Enzyme Inhibitors - pharmacology; therapeutic use
Animals
Cyclin-Dependent Kinase Inhibitor p27 - biosynthesis; genetics
Enalapril - pharmacology; therapeutic use
Female
Kidney Tubules - metabolism

Nephrosclerosis - drug therapy; etiology; metabolism
RNA, Messenger - biosynthesis; genetics
Random Allocation
Rats
Rats, Sprague-Dawley
Ureteral Obstruction - complications

Associated Chemicals: Angiotensin-Converting Enzyme Inhibitors (0) ; RNA, Messenger (0) ; Cyclin-Dependent Kinase Inhibitor p27 (147604-94-2) ; Enalapril (75847-73-3)

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