Fine needle aspiration of the vocal fold lamina propria in an animal model.

Full Abstract

OBJECTIVES:
The development of a minimally invasive fine needle aspiration (FNA) technique for vocal fold (VF) biopsy would have far-reaching implications and applicability in laryngology. The objective of this study was to determine whether FNA of the VF lamina propria is feasible and whether it causes injury to the VF.

METHODS:
Unilateral VF FNA was performed with a 26-gauge needle on 20 rabbits. The FNA cell collection of the lamina propria was confirmed by cytology. Four weeks after the FNA, the rabbits were painlessly sacrificed and the larynges were harvested. Histologic analysis of the VF lamina propria included Masson's trichrome and elastin-van Gieson stains. The linear viscoelastic shear properties, elastic modulus, and viscous modulus of the tissue were measured.

RESULTS:
Compared to the contralateral normal VFs, those VFs that underwent FNA demonstrated no significant differences in levels of collagen (p = .17) and elastin (p = .94). Rheologically, the elastic shear modulus and viscous modulus did not significantly differ between the normal and FNA VFs (p = .9380 and p = .9359, respectively).

CONCLUSIONS:
Fine needle aspiration of the VF lamina propria is feasible without injury and offers a potentially promising, less invasive alternative to be developed for future diagnostic and therapeutic management of VF lesions.

Learn Faster Today Improve your study skills

Author information

Author/s: Cobell, Will (W); Duflo, Suzy M (SM); Magrufov, Akhmar (A); Thibeault, Susan L (SL);

Affiliation: Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, School of Medicine, University of Utah, Salt Lake City, USA.

Grants: R01 DC4336 (Agency:NIDCD NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural

Journal: The Annals of otology, rhinology, and laryngology (Ann Otol Rhinol Laryngol), published in United States. (Language: eng)

Reference: 2006-Oct; vol 115 (issue 10) : pp 764-8

Dates: Created 2006/11/01; Completed 2006/11/28; Revised 2007/12/03;

PMID: 17076099, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Biopsy, Fine-Needle - adverse effects
Collagen - metabolism
Elastin - metabolism
Feasibility Studies

Feasibility Studies
Models, Animal
Mucous Membrane - metabolism; pathology
Rabbits
Vocal Cords - cytology; metabolism; pathology

Associated Chemicals: Collagen (9007-34-5) ; Elastin (9007-58-3)

These are the highest related articles currently in the database:

See 100+ related articles.