Research article summary (published 30 Aug 2006):

Influence of quercetin and x-ray on collagen synthesis of cultured human keloid-derived fibroblasts.

Full Abstract

OBJECTIVE: To investigate the effects of quercetin and X-ray on collagen synthesis of cultured human keloid-derived fibroblast and the mechanism. METHODS: Collagen synthesis of cultured human keloid and normal fibroblasts were detected by hydroxyproline colorimetric analysis. Immunocytochemical staining was used to investigate collagen I and III expression. mRNA expression of collagen I and III, and transforming growth factor (TGF)-beta 1 were assayed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. RESULTS: Quercetin inhibited the collagen synthesis of both keloid and normal fibroblasts in a dose-dependent manner. Immunocytochemical staining indicated that collagen I and III were down-regulated by quercetin and X-ray (P < 0.05), particularly collagen I (P < 0.05). mRNA expression of both collagen I and III in quercetin groups significantly decreased compared with that in control group (P < 0.05), especially in the group treated with both quercetin and X-ray (P < 0.01). mRNA level of TGF-beta 1 gene was down-regulated by quercertin (P < 0.05). CONCLUSIONS: Quercetin will probably be one of the new medicines which could effectively treat keloid. Quercetin combined with X-ray could reduce the dose of radiation.

Author information

Author/s: Long, Xiao (X); Zeng, Xuan (X); Zhang, Fu-quan (FQ); Wang, Xiao-jun (XJ);

Affiliation: Plastic & Aesthetic Surgery Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730.

Journal and publication information

Publication Type: Journal Article

Journal: Chinese medical sciences journal = Chung-kuo i hsüeh k'o hsüeh tsa chih / Chinese Academy of Medical Sciences (Chin Med Sci J), published in China. (Language: eng)

Reference: 2006-Sep; vol 21 (issue 3) : pp 179-83

Dates: Created 2006/11/07; Completed 2007/09/14;

PMID: 17086741, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult Cells, Cultured Collagen Type I - biosynthesis; genetics; radiation effects Collagen Type III - biosynthesis; genetics; radiation effects Dose-Response Relationship, Drug Female Fibroblasts - metabolism; pathology; radiation effects

Humans Keloid - metabolism; pathology Male Quercetin - administration & dosage; pharmacology RNA, Messenger - biosynthesis Skin - cytology Transforming Growth Factor beta1 - biosynthesis; genetics; radiation effects

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; RNA, Messenger (0) ; Transforming Growth Factor beta1 (0) ; Quercetin (117-39-5)

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