Determination of ethyl sulfate--a marker for recent ethanol consumption--in human urine by CE with indirect UV detection.

Full Abstract

A CE method for the determination of the ethanol consumption marker ethyl sulfate (EtS) in human urine was developed. Analysis was performed in negative polarity mode with a background electrolyte composed of 15 mM maleic acid, 1 mM phthalic acid, and 0.05 mM cetyltrimethylammonium bromide (CTAB) at pH 2.5 and indirect UV detection at 220 nm (300 nm reference wavelength). This buffer system provided selective separation conditions for EtS and vinylsulfonic acid, employed as internal standard, from urine matrix components. Sample pretreatment of urine was minimized to a 1:5 dilution with water. The optimized CE method was validated in the range of 5-700 mg/L using seven lots of urine. Intra- and inter-day precision and accuracy values, determined at 5, 60, and 700 mg/L with each lot of urine, fulfilled the requirements according to common guidelines for bioanalytical method validation. The application to forensic urine samples collected at autopsies as well as a successful cross-validation with a LC-MS/MS-based method confirmed the overall validity and real-world suitability of the developed expeditious CE assay (sample throughput 130 per day).

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Author information

Author/s: Esteve-Turrillas, Francesc A (FA); Bicker, Wolfgang (W); Lämmerhofer, Michael (M); Keller, Thomas (T); Lindner, Wolfgang (W);

Affiliation: Analytical Chemistry Department, University of Valencia, Burjassot, Spain.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies

Journal: Electrophoresis (Electrophoresis), published in Germany. (Language: eng)

Reference: 2006-Dec; vol 27 (issue 23) : pp 4763-71

Dates: Created 2006/12/04; Completed 2007/03/13;

PMID: 17091466, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult
Alcohol Drinking - urine
Biological Markers - urine
Buffers
Cetrimonium Compounds - chemistry
Chromatography, Liquid
Electrophoresis, Capillary - methods
Ethanol - administration & dosage

Female
Forensic Toxicology - methods
Humans
Male
Maleates - chemistry
Phthalic Acids - chemistry
Spectrophotometry, Ultraviolet - methods
Sulfuric Acid Esters - urine

Associated Chemicals: Biological Markers (0) ; Buffers (0) ; Cetrimonium Compounds (0) ; Maleates (0) ; Phthalic Acids (0) ; Sulfuric Acid Esters (0) ; maleic acid (110-16-7) ; Ethanol (64-17-5) ; diethyl sulfate (64-67-5) ; cetrimonium (6899-10-1) ; phthalic acid (88-99-3)

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