Determination of active components of Ginkgo biloba in human urine by capillary high-performance liquid chromatography/mass spectrometry with on-line column-switching purification.

Full Abstract

Ginkgo biloba is one of the most popular herbal nutritional supplements, with terpene lactones and flavonoids being the two major active components. An on-line purification high-performance liquid chromatography/mass spectrometry (HPLC/MS) method was successfully developed for the quantitative determination of flavonoids and terpene lactones excreted in human urine after ingesting the herbal supplement. Satisfactory separation was obtained using a C18 capillary column made in-house with sample clean-up and pre-concentration achieved using a C18 pre-column with column switching. High selectivity and limits of detection of 1-18 ng/mL were achieved using a selected ion monitoring (SIM) scan in negative ion mode; the on-line solid-phase extraction (SPE) recovery of the active components in Ginkgo biloba determined in this study was greater than 75%.Copyright 2006 John Wiley & Sons, Ltd.

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Author information

Author/s: Ding, Shujing (S); Dudley, Ed (E); Chen, Lijuan (L); Plummer, Sue (S); Tang, Jiandong (J); Newton, Russell P (RP); Brenton, A Gareth (AG);

Affiliation: Mass Spectrometry Research Unit, Grove Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Validation Studies

Journal: Rapid communications in mass spectrometry : RCM (Rapid Commun Mass Spectrom), published in England. (Language: eng)

Reference: 2006-; vol 20 (issue 24) : pp 3619-24

Dates: Created 2006/11/30; Completed 2007/01/30;

PMID: 17094168, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Capillary Electrochromatography - instrumentation; methods
Chromatography, High Pressure Liquid - instrumentation; methods
Flavonoids - urine
Ginkgo biloba - chemistry
Humans

Mass Spectrometry - instrumentation; methods
Plant Extracts - urine
Reproducibility of Results
Sensitivity and Specificity
Urinalysis - instrumentation; methods

Associated Chemicals: Flavonoids (0) ; Plant Extracts (0)

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