Competitive immunoassay for clenbuterol using capillary electrophoresis with laser-induced fluorescence detection.

Full Abstract

A competitive immunoassay for detecting clenbuterol in urine was established by capillary electrophoresis (CE) with laser-induced fluorescence (LIF). The clenbuterol was conjugated with bovine serum albumin (BSA), and then the derivative was labeled with fluorescein isothiocyanate (FITC) and competes for antibody with free clenbuterol in the sample. Under the optimal conditions, Free and bound FITC labeled clenbuterol was separated within 8 min with the relative standard deviation (R.S.D.) 0.72% for migration time and 2.8% for peak area. The detection limit reached 0.7 ng/ml.

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Author information

Author/s: Zhou, Jieyu (J); Xu, Xueshu (X); Wang, Yuxiao (Y);

Affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 283, Shanghai 200237, PR China.

Journal and publication information

Publication Type: Journal Article

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci), published in Netherlands. (Language: eng)

Reference: 2007-Apr; vol 848 (issue 2) : pp 226-31

Dates: Created 2007/03/27; Completed 2007/06/11;

PMID: 17097930, status: MEDLINE (last retrieval date: 11/6/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Cattle
Clenbuterol - analysis; chemistry
Electrophoresis, Capillary - methods
Enzyme-Linked Immunosorbent Assay
Fluorescence

Fluorescence
Immunoassay - methods
Isothiocyanates - chemistry
Lasers
Reproducibility of Results
Serum Albumin, Bovine - chemistry

Associated Chemicals: Isothiocyanates (0) ; Serum Albumin, Bovine (0) ; isothiocyanic acid (3129-90-6) ; Clenbuterol (37148-27-9)

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