Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I.

Full Abstract

The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca(2+), Mg(2+), Mn(2+) and Ni(2+)) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified.

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Author information

Author/s: Stettler, Alexandra R (AR); Chaurin, Valérie (V); Constable, Edwin C (EC); Housecroft, Catherine E (CE); Schwarz, Maria A (MA);

Affiliation: Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry (J Biol Inorg Chem), published in Germany. (Language: eng)

Reference: 2007-Feb; vol 12 (issue 2) : pp 194-203

Dates: Created 2007/01/31; Completed 2007/04/10;

PMID: 17120074, status: MEDLINE (last retrieval date: 11/6/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Calcium - chemistry
Cations, Divalent - chemistry
DNA - chemistry
Electrophoresis, Capillary - methods
Magnesium - chemistry
Manganese - chemistry

Metals - chemistry
Morpholines - chemistry
Nickel - chemistry
Nucleic Acids - chemistry
Oligonucleotides - chemistry
Tromethamine - chemistry

Associated Chemicals: Cations, Divalent (0) ; Metals (0) ; Morpholines (0) ; Nucleic Acids (0) ; Oligonucleotides (0) ; morpholinopropane sulfonic acid (1132-61-2) ; Magnesium (7439-95-4) ; Manganese (7439-96-5) ; Nickel (7440-02-0) ; Calcium (7440-70-2) ; Tromethamine (77-86-1) ; DNA (9007-49-2)

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