Research article summary (published 19 Dec 2006):

Increased insulin-stimulated Akt pSer473 and cytosolic SHP2 protein abundance in human skeletal muscle following acute exercise and short-term training.

Full Abstract

The purpose of the present study was to determine in human skeletal muscle whether a single exercise bout and 7 days of consecutive endurance (cycling) training 1) increased insulin-stimulated Akt pSer(473) and 2) altered the abundance of the protein tyrosine phosphatases (PTPases), PTP1B and SHP2. In healthy, untrained men (n = 8; 24 +/- 1 yr), glucose infusion rate during a hyperinsulinemic euglycemic clamp, when compared with untrained values, was not improved 24 h following a single 60-min bout of endurance cycling but was significantly increased ( approximately 30%; P < 0.05) 24 h following completion of 7 days of exercise training. Insulin-stimulated Akt pSer(473) was approximately 50% higher (P < 0.05) 24 h following the acute bout of exercise, with this effect remaining after 7 days of training (P < 0.05). Insulin-stimulated insulin receptor and insulin receptor substrate-1 tyrosine phosphorylation were not altered 24 h after acute exercise and short-term training. Insulin did not acutely regulate the localization of the PTPases, PTP1B or SHP2, although cytosolic protein abundance of SHP2 was increased (P < 0.05; main effect) 24 h following acute exercise and short-term training. In conclusion, insulin-sensitive Akt pSer(473) and cytosolic SHP2 protein abundance are higher after acute exercise and short-term training, and this effect appears largely due to the residual effects of the last bout of prior exercise. The significance of exercise-induced alterations in cytosolic SHP2 and insulin-stimulated Akt pSer(473) on the improvement in insulin sensitivity requires further elucidation.

Author information

Author/s: Wadley, Glenn D (GD); Konstantopoulos, Nicky (N); Macaulay, Lance (L); Howlett, Kirsten F (KF); Garnham, Andrew (A); Hargreaves, Mark (M); Cameron-Smith, David (D);

Affiliation: 1School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia. gdwadley(-atsign-)unimelb.edu.au

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of applied physiology (Bethesda, Md. : 1985) (J Appl Physiol), published in United States. (Language: eng)

Reference: 2007-Apr; vol 102 (issue 4) : pp 1624-31

Dates: Created 2007/04/05; Completed 2007/05/22; Revised 2007/11/15;

PMID: 17185494, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adaptation, Physiological - drug effects; physiology Adult Cytosol - metabolism Exercise - physiology Humans Insulin - administration & dosage Intracellular Signaling Peptides and Proteins - metabolism Male

Muscle, Skeletal - drug effects; physiology Phosphorylation Physical Endurance - drug effects; physiology Physical Fitness - physiology Protein Tyrosine Phosphatase, Non-Receptor Type 1 Protein Tyrosine Phosphatase, Non-Receptor Type 11 Protein Tyrosine Phosphatases - metabolism Proto-Oncogene Proteins c-akt - metabolism

Associated Chemicals: Intracellular Signaling Peptides and Proteins (0) ; Insulin (11061-68-0) ; Proto-Oncogene Proteins c-akt (EC 2.7.1.37) ; PTPN1 protein, human (EC 3.1.3.48) ; PTPN11 protein, human (EC 3.1.3.48) ; Protein Tyrosine Phosphatase, Non-Receptor Type 1 (EC 3.1.3.48) ; Protein Tyrosine Phosphatase, Non-Receptor Type 11 (EC 3.1.3.48) ; Protein Tyrosine Phosphatases (EC 3.1.3.48)

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