Sensitive determination of erythrosine and other red food colorants using capillary electrophoresis with laser-induced fluorescence detection.

Full Abstract

Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was applied to separation and sensitive determination of red food colorants. Diode pumped frequency-doubled Nd:YAG laser (532 nm) was used as an excitation source in a laboratory-built CE-LIF system. For highly fluorescent erythrosine B (E127), an extrapolated limit of detection (LOD) of 0.4 ng mL(-1) (S/N=3) was achieved. Extrapolated LODs of other tested red additives, such as carmoisine, E122 (0.5 microg mL(-1)); amaranth, E123 (0.2 microg mL(-1)); ponceau 4R, E124 (0.3 microg mL(-1)) and red 2G, E128 (0.3 microg mL(-1)) were about one-order lower compared to results obtained with CE with absorbance detection in UV/vis (CE-UV/vis). The main advantages of using CE-LIF for analysis of food samples are high selectivity and minimization of matrix effect. To our knowledge, this is the first use of CE-LIF for determination of red food colorants.

Learn Faster Today Improve your study skills

Author information

Author/s: Ryvolová, Markéta (M); Táborský, Petr (P); Vrábel, Patrik (P); Krásenský, Pavel (P); Preisler, Jan (J);

Affiliation: Department of Analytical Chemistry, Faculty of Science, Masaryk University, Kotlárská 2, 611 37 Brno, Czech Republic.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of chromatography. A (J Chromatogr A), published in Netherlands. (Language: eng)

Reference: 2007-Feb; vol 1141 (issue 2) : pp 206-11

Dates: Created 2007/01/22; Completed 2007/04/09;

PMID: 17188280, status: MEDLINE (last retrieval date: 11/6/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Coloring Agents - analysis
Electrophoresis, Capillary - methods
Erythrosine - analysis
Food Analysis

Food Analysis
Lasers
Sensitivity and Specificity
Spectrometry, Fluorescence - methods

Associated Chemicals: Coloring Agents (0) ; Erythrosine (16423-68-0)

These are the highest related articles currently in the database:

See 100+ related articles.