The RNA-binding protein Sam68 contributes to proliferation and survival of human prostate cancer cells.

Full Abstract

The tyrosine kinase Src is frequently activated in advanced human prostate carcinomas and its activation correlates with tyrosine phosphorylation of the RNA-binding protein Sam68. Herein, we have investigated the expression and function of Sam68 in human prostate cancer cells. Analysis of specimens obtained from 20 patients revealed that Sam68 is upregulated at the protein level in 35% of the samples. Real-time polymerase chain reaction confirmed the results at the mRNA level in most patients. Downregulation of Sam68 by RNAi in LNCaP prostate cancer cells delayed cell cycle progression and reduced the proliferation rate. Moreover, depletion of Sam68 sensitized cells to apoptosis induced by DNA-damaging agents. Similarly, stable cell lines expressing a truncated GFP-Sam68(GSG) protein displayed reduced growth rates and higher sensitivity to cisplatin-induced apoptosis. Microarray analyses revealed that a subset of genes involved in proliferation and apoptosis were altered when Sam68 was knocked down in LNCaP cells. Our results indicate that Sam68 expression supports prostate cancer cells proliferation and survival to cytotoxic agents.

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Author information

Author/s: Busà, R (R); Paronetto, M P (MP); Farini, D (D); Pierantozzi, E (E); Botti, F (F); Angelini, D F (DF); Attisani, F (F); Vespasiani, G (G); Sette, C (C);

Affiliation: Department of Public Health and Cell Biology, University of Rome Tor Vergata, Rome, Italy.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Oncogene (Oncogene), published in England. (Language: eng)

Reference: 2007-Jun; vol 26 (issue 30) : pp 4372-82

Dates: Created 2007/06/28; Completed 2007/08/02;

PMID: 17237817, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adaptor Proteins, Signal Transducing - chemistry; genetics; physiology
Aged
Apoptosis
Cell Line, Tumor
Cell Proliferation
Cell Survival
DNA-Binding Proteins - chemistry; genetics; physiology

Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Male
Middle Aged
Prostatic Neoplasms - metabolism; pathology
RNA-Binding Proteins - chemistry; genetics; physiology

Associated Chemicals: Adaptor Proteins, Signal Transducing (0) ; DNA-Binding Proteins (0) ; KHDRBS1 protein, human (0) ; RNA-Binding Proteins (0)

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