[Megakaryopoietic cytokine levels in patients with essential thrombocythemia and their relationship with clinical and biochemical features]

(Niveles de citoquinas megacariocitopoyeticas en pacientes con trombocitemia esencial y su relacion con caracteristicas clinicas y bioquimicas.)

Full Abstract

Megakaryopoiesis and platelet production are driven by transcription factors and cytokines present in bone marrow environment. Essential thrombocythemia (ET) is a chronic myeloproliferative disorder characterized by high platelet count and megakaryocytic hyperplasia. In the present work we evaluated plasmatic levels of cytokines involved in megakaryocytic development in a group of patients with ET that were not on treatment, as well as thrombopoietin (TPO) levels before and during anagrelide treatment. The assays were carried out using ELISA techniques. Among the cytokines mainly involved in proliferation of megakaryocytic progenitors, interleukin 3 (IL-3) levels were found increased in patients compared to normal controls (p = 0.0383). Granulocyte-macrophage colony stimulating factor and stem cell factor levels were normal. Interleukin 6, as well as interleukin 11 and erythropoietin (EPO), cytokines mainly related to megakaryocytic maturation, were normal. Plasma TPO levels before treatment were within the normal range and increased during treatment but the difference was not statistically significant. Patients who displayed spontaneous platelet aggregation had higher plasma TPO levels compared to those who did not (p = 0.049). We did not find any relationship between cytokine levels and clinical or laboratory parameters. The high IL-3 levels seen in some patients with ET could contribute to megakaryocytic proliferation. The simultaneous occurrence of higher TPO levels and elevated platelet count could be a predisposing factor for the development of spontaneous platelet aggregation in ET patients.

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Author information

Author/s: Marta, Rosana F (RF); Goette, Nora P (NP); Molinas, Felisa C (FC);

Affiliation: Sección Hematología Investigación, Instituto de Investigaciones Médicas Alfredo Lanari, Facultad de Medicina, Universidad de Buenos Aires, Argentina. rfmarta(-atsign-)ciudad.com.ar

Journal and publication information

Publication Type: Controlled Clinical Trial; English Abstract; Journal Article

Journal: Medicina (Medicina (B Aires)), published in Argentina. (Language: spa)

Reference: 2006-; vol 66 (issue 6) : pp 540-6

Dates: Created 2007/01/23; Completed 2007/08/16; Revised 2008/11/21;

PMID: 17240625, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Enzyme-Linked Immunosorbent Assay
Granulocyte-Macrophage Colony-Stimulating Factor - blood; drug effects
Humans
Interleukin-3 - blood
Megakaryocytes - drug effects; physiology
Platelet Aggregation - drug effects; physiology
Platelet Aggregation Inhibitors - therapeutic use
Quinazolines - therapeutic use

Quinazolines - therapeutic use
Retrospective Studies
Statistics, Nonparametric
Stem Cell Factor - blood; drug effects
Thrombocythemia, Essential - blood; drug therapy
Thrombocytosis - chemically induced
Thrombopoiesis - drug effects; physiology
Thrombopoietin - blood; drug effects

Associated Chemicals: Interleukin-3 (0) ; Platelet Aggregation Inhibitors (0) ; Quinazolines (0) ; Stem Cell Factor (0) ; anagrelide (0) ; Granulocyte-Macrophage Colony-Stimulating Factor (83869-56-1) ; Thrombopoietin (9014-42-0)

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